BrdU (Bromodeoxyuridine)

Catalog No.S7918 Batch:S791805

Technical Data

Formula	C₉H₁₁BrN₂O₅
Molecular Weight	307.1	CAS No.	59-14-3
Solubility (25°C)*	In vitro	DMSO	61 mg/mL (198.63 mM)
		Ethanol	2 mg/mL (6.51 mM)
		Water	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description	BrdU (Bromodeoxyuridine) is a nucleoside analog that competes with thymidine for incorporation into DNA, and used in the detection of proliferating cells.
In vitro	In RG2 rat glioma cells, Bromodeoxyuridine induces a progressive, dose-responsive suppression of cancer cell line and cancer stem cell population expansion. In H9 cells and BJ fibroblasts, Bromodeoxyuridine alters the cell cycle profile. ^[1] BrdU is stably integrated into the DNA, and thus can be used in assessment of cell proliferation and other cell procession. ^[2]
In vivo	In rat glioma RG2 tumor model, Bromodeoxyuridine (300 mg/kg, i.p. or 0.8 mg/ml, p.o.) significantly slows tumor progression. ^[1]

Description

BrdU (Bromodeoxyuridine) is a nucleoside analog that competes with thymidine for incorporation into DNA, and used in the detection of proliferating cells.

In vitro

In RG2 rat glioma cells, Bromodeoxyuridine induces a progressive, dose-responsive suppression of cancer cell line and cancer stem cell population expansion. In H9 cells and BJ fibroblasts, Bromodeoxyuridine alters the cell cycle profile. ^[1]

BrdU is stably integrated into the DNA, and thus can be used in assessment of cell proliferation and other cell procession. ^[2]

In vivo

In rat glioma RG2 tumor model, Bromodeoxyuridine (300 mg/kg, i.p. or 0.8 mg/ml, p.o.) significantly slows tumor progression. ^[1]

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines RG2 rat glioma cells Concentrations ~ 50 µM Incubation Time 24 hours Method Cultures are initially plated at 2000 cells/cm² and are quantified with a Z2 Coulter Counter. RG2 rat glioma cells are treated once with 0, 1, 10, or 50 µM BrdU for 24 hours, and cumulative growth curves were obtained over 18 days. Control and treated cells are quantified and replated at equal densities on days 5, 12, and 18 after treatment.

Cell Assay:^{^[1]}

Cell lines
RG2 rat glioma cells
Concentrations
~ 50 µM
Incubation Time
24 hours
Method
Cultures are initially plated at 2000 cells/cm² and are quantified with a Z2 Coulter Counter. RG2 rat glioma cells are treated once with 0, 1, 10, or 50 µM BrdU for 24 hours, and cumulative growth curves were obtained over 18 days. Control and treated cells are quantified and replated at equal densities on days 5, 12, and 18 after treatment.

References

Customer Product Validation

: Data from [Data independently produced by , , PLoS One, 2016, 11(11):e0166386.]

: Data from [Data independently produced by , , PLoS One, 2016, 11(11):e0166386]

: Data from [Data independently produced by , , Int J Ophthalmol, 2017, 10(4):507-514]

Selleck's BrdU (Bromodeoxyuridine) has been cited by 14 publications

Hippocampal mitophagy contributes to spatial memory via maintaining neurogenesis during the development of mice [ CNS Neurosci Ther, 2024, 30(6):e14800]	PubMed: 38887162
Astragaloside IV attenuates sunitinib-associated cardiotoxicity by inhibiting COUP-TFII [ Heliyon, 2024, 10(3):e24779]	PubMed: 38314260
Survivin inhibition attenuates EGF-induced epithelial mesenchymal transformation of human RPE cells via the EGFR/MAPK pathway [ PLoS One, 2024, 19(8):e0309539]	PubMed: 39213375
Inhibition of EGFR attenuates EGF-induced activation of retinal pigment epithelium cell via EGFR/AKT signaling pathway [ Int J Ophthalmol, 2024, 17(6):1018-1027]	PubMed: 38895677
miR-432-5p Inhibits the Ferroptosis in Cardiomyocytes Induced by Hypoxia/Reoxygenation via Activating Nrf2/SLC7A11 Axis by Degrading Keap1 [ Anal Cell Pathol -Amst), 2023, 2023:1293200]	PubMed: 37822721
CDCA8, targeted by MYBL2, promotes malignant progression and olaparib insensitivity in ovarian cancer [ Am J Cancer Res, 2021, 11(2):389-415]	PubMed: 33575078
Activation of 6-8-week-old new mature adult-born dentate granule cells contributes to anxiety-like behavior [ Neurobiol Stress, 2021, 15:100358]	PubMed: 34195305
YM155 inhibits retinal pigment epithelium cell survival through EGFR/MAPK signaling pathway [ Int J Ophthalmol, 2021, 14(4):489-496]	PubMed: 33875937
lncRNA Oip5-as1 Attenuates Myocardial Ischaemia/Reperfusion Injury by Sponging miR-29a to Activate the SIRT1/AMPK/PGC1α Pathway [ Cell Prolif, 2020, 28;e12818]	PubMed: 32468629
Neferine inhibits growth and migration of gastrointestinal stromal tumor cell line GIST-T1 by up-regulation of miR-449a. [ Biomed Pharmacother, 2019, 109:1951-1959]	PubMed: 30551450

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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