BRD3308

Catalog No.S8962 Batch:S896201

Technical Data

Formula

C₁₅H₁₄FN₃O₂

Molecular Weight

287.29

CAS No.

1550053-02-5

Solubility (25°C)*

In vitro

DMSO

57 mg/mL (198.4 mM)

Water

Insoluble

Ethanol

Insoluble

In vivo (Add solvents to the product individually and in order)

Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O	2.85mg/ml	Taking the 1 mL working solution as an example, add 50 μL of 57 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil	0.35mg/ml	Taking the 1 mL working solution as an example, add 50 μL of 7 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description

BRD3308 is a potant and highly selective inhibitor of HDAC3 with IC50 of 54 nM, 1.26 μM and 1.34 μM for HDAC3, HDAC1 and HDAC2, respectively. BRD3308 activates HIV-1 transcription. BRD3308 suppresses pancreatic β-cell apoptosis induced by inflammatory cytokines (glucolipotoxic stress) and increases functional insulin release.

Targets

HDAC3 ^[1] (Cell-free assay)	HDAC1 ^[1] (Cell-free assay)	HDAC2 ^[1] (Cell-free assay)
54 nM	1.26 μM	1.34 μM

In vitro

BRD3308 is a derivative of the ortho-aminoanilide HDAC inhibitor CI-994 and is developed to be highly selective for inhibition of HDAC3 with an IC50 value that is 23-fold lower for HDAC3 than for HDAC1 or 22. HDAC3 selective inhibition induces expression of HIV and exposure to BRD3308 allows the recovery of latent HIV-1 from patient cells.^[1]

In vivo

Selective inhibition of HDAC3 by BRD3308 prevents diabetes onset in female NOD mice. HDAC3 treatment in vivo prevents pancreatic islet infiltration and protects β-cells from apoptosis. β-cell proliferation is increased in animals treated with BRD3308. HDAC3 treatment in vivo prevents white adipose tissue infiltration in NOD mice.^[2]

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines 2D10 cells, J89 cells, THP89 cells, J-Lat 6.3 cells Concentrations 5 μM, 10 μM, 15 μM, 30 μM Incubation Time 6 h, 12 h, 18 h, 24 h Method BRD3308 (15 μM) is added to aliquots of resting CD4+ T cells which are then plated in limiting dilution replicates of 2.5 or 1 million, 0.5 million, and 0.1 million cells and incubated at 37℃ under 5% CO₂. BRD3308 is removed from the cultures after 24 hours.
Animal Study:^{^[2]}	Animal Models Female NOD/ShiLTJ (NOD) mice Dosages 0.1 mg/kg, 1 mg/kg, 10 mg/kg Administration IP

Cell Assay:^{^[1]}

Cell lines
2D10 cells, J89 cells, THP89 cells, J-Lat 6.3 cells
Concentrations
5 μM, 10 μM, 15 μM, 30 μM
Incubation Time
6 h, 12 h, 18 h, 24 h
Method
BRD3308 (15 μM) is added to aliquots of resting CD4+ T cells which are then plated in limiting dilution replicates of 2.5 or 1 million, 0.5 million, and 0.1 million cells and incubated at 37℃ under 5% CO₂. BRD3308 is removed from the cultures after 24 hours.

Animal Study:^{^[2]}

Animal Models
Female NOD/ShiLTJ (NOD) mice
Dosages
0.1 mg/kg, 1 mg/kg, 10 mg/kg
Administration
IP

References

RETURN POLICY
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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.