bpV (HOpic)

Catalog No.S8651 Batch:S865101

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Technical Data

Formula

C6H4NO8V.2K
Molecular Weight 347.24 CAS No. 722494-26-0
Solubility (25°C)* In vitro Water 69 mg/mL (198.7 mM)
DMSO Insoluble
Ethanol Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description bpV (HOpic) (Bisperoxovanadium (HOpic)) is a potent inhibitor of PTEN with an IC50 of 14 nM. The IC50s for PTP-β and PTP-1B are about 350- and 1800-fold higher than the IC50 for PTEN, respectively.
Targets
PTEN [1]
(Cell-free assay)
14 nM
In vitro 1 μM BpV(Hopic) treatment is capable of increasing the in vitro migration of C2C12 myoblasts, without significantly reducing their ability to differentiate and fuse into multinucleated myotubes. The effects on myoblast migration is in conjuction with enhanced AKT and ERK1/2 signaling[2].
In vivo Pharmacological inhibition of PTEN with bpV(HOpic) exacerbates renal dysfunction and promotes tubular damage in mice with IRI(renal ischemia/reperfusion injury) compared with vehicle-treated mice with IRI. PTEN inhibition enhances tubular cell apoptosis in kidneys with IRI, which is associated with excessive caspase-3 activation. Furthermore, PTEN inhibition expands the infiltration of neutrophils and macrophages into kidneys with IRI, which is accompanied by increased expression of the proinflammatory molecules[3].

Protocol (from reference)

Cell Assay:

[2]
  • Cell lines

    C2C12 myoblasts

  • Concentrations

    1 μM or 10 μM

  • Incubation Time

    72 h

  • Method

    C2C12 myoblasts are grown to high confluency and triggered to differentiate in the presence of DM, supplemented with 1 μM or 10 μM BpV(Hopic).
Animal Study:

[3]
  • Animal Models

    mouse model of ischemia-reperfusion injury (C57BL/6 mice, 8-12 weeks old)

  • Dosages

    200 μg/kg

  • Administration

    i.p.

Selleck's bpV (HOpic) has been cited by 19 publications

Pten regulates endocytic trafficking of cell adhesion and Wnt signaling molecules to pattern the retina [ Cell Rep, 2024, 43(4):114005] PubMed: 38551961
SET8 inhibition preserves PTEN to attenuate kidney cell apoptosis in cisplatin nephrotoxicity [ Res Sq, 2024, rs.3.rs-4603170] PubMed: 39184108
Single-cell analysis reveals an Angpt4-initiated EPDC-EC-CM cellular coordination cascade during heart regeneration [ Protein Cell, 2023, 14(5):350-368] PubMed: 37155312
Malic Enzyme 1 as a Novel Anti-Ferroptotic Regulator in Hepatic Ischemia/Reperfusion Injury [ Adv Sci (Weinh), 2023, 10(13):e2205436] PubMed: 36840630
Downregulation of KLF10 contributes to the regeneration of survived renal tubular cells in cisplatin-induced acute kidney injury via ZBTB7A-KLF10-PTEN axis [ Cell Death Discov, 2023, 9(1):82] PubMed: 36878898
The anti-angiogenesis mechanism of Geniposide on rheumatoid arthritis is related to the regulation of PTEN [ Inflammopharmacology, 2022, 30(3):1047-1062] PubMed: 35389123
Primary Cilia Restrain PI3K-AKT Signaling to Orchestrate Human Decidualization [ Int J Mol Sci, 2022, 23(24)15573] PubMed: 36555215
Primary Cilia Restrain PI3K-AKT Signaling to Orchestrate Human Decidualization [ Int J Mol Sci, 2022, 23(24)15573] PubMed: 36555215
Geniposide restricts angiogenesis in experimentary arthritis via inhibiting Dnmt1-mediated PTEN hypermethylation [ Int Immunopharmacol, 2022, 111:109087] PubMed: 35908504
Progerin modulates the IGF-1R/Akt signaling involved in aging [ Sci Adv, 2022, 8(27):eabo0322] PubMed: 35857466

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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