BOS172722

Catalog No.S8911 Batch:S891101

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Technical Data

Formula

C24H30N8O
Molecular Weight 446.55 CAS No. 1578245-44-9
Solubility (25°C)* In vitro DMSO 89 mg/mL (199.3 mM)
Water Insoluble
Ethanol Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description BOS172722 is a monopolar spindle 1 (MPS1) checkpoint inhibitor with IC50 of 11 nM, Ki of 0.11 nM.
Targets
MPS1 [1]
(Cell-free assay)		 MPS1 [1]
(Cell-free assay)
0.11 nM(Ki) 11 nM

Biological Activity

Description BOS172722 is a monopolar spindle 1 (MPS1) checkpoint inhibitor with IC50 of 11 nM, Ki of 0.11 nM.
Targets
MPS1 [1]
(Cell-free assay)		 MPS1 [1]
(Cell-free assay)
0.11 nM(Ki) 11 nM
In vitro

BOS172722 optimizes HLM stability, CDK2 selectivity, cellular potency, and solubility. BOS172722 is potential for the treatment of various forms of breast cancer.[1]
In vivo

BOS172722 shows excellent PK in mouse, rat, and dog. At 1 mg/kg iv and 5 mg/kg po, BOS172722 shows complete bioavailability (100%), low clearance (1.2 mL/min/kg), a moderate volume of distribution (1.1 L/kg), and a 12 h half-life in a dog PK study. The 50 mg/kg dose of BOS172722 results in an increased suppression of MPS1 autophosphorylation at 6 and 24 h.[1]

Protocol (from reference)

Cell Assay:

[1]
  • Cell lines

    CAL51 cell line

  • Concentrations

    --

  • Incubation Time

    72 h

  • Method

    GI50 are determined using the colorimetric 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay according to manufacturer's instructions. Cells are plated in 96-well plates at 2,500 per well and treated for 72 h. The optical density is measured at 570 nm using the Wallac VICTOR2TM 1420 Multilabel Counter.
Animal Study:

[1]
  • Animal Models

    Dog, mouse, rat

  • Dosages

    1 mg/kg, 5 mg/kg, 2.5 mg/kg

  • Administration

    IV, PO

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Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.

SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.