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Formula | C18H16N2O2 |
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Molecular Weight | 292.33 | CAS No. | 856925-71-8 | |
Solubility (25°C)* | In vitro | DMSO | 58 mg/mL (198.4 mM) | |
Water | Insoluble | |||
Ethanol | Insoluble | |||
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.) |
Description | (-)-Blebbistatin ((S)-(-)-Blebbistatin) is a cell-permeable inhibitor for non muscle myosin II ATPase with IC50 of ~2 μM in cell-free assays, does not inhibit myosin light chain kinase, inhibits contraction of the cleavage furrow without disrupting mitosis or contractile ring assembly. | ||
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In vitro | Blebbistatin, inhibits cytokinesis, alters the smooth movement of fish keratocytes and inhibits the spontaneous blebbing of a cell line lacking filamin. Blebbistatin is shown to inhibit the enzymatic activities of HMM fragments of nonmuscle myosin IIA, nonmuscle myosin IIB and of rabbit skeletal muscle myosin S1, but not smooth muscle myosin[1] Blebbistatin rapidly and reversibly inhibits Mg-ATPase activity and in vitro motility of non-muscle myosin IIA and IIB for several species, while poorly inhibiting smooth muscle myosin (IC50 = 80 μM). Blebbistatin potently inhibits Dictyostelium myosin II, but poorly inhibits Acanthamoeba myosin II. Blebbistatin does not inhibit representative myosin superfamily members from classes I, V, and X. [2] Blebbistatin does not compete with nucleotide binding to the skeletal muscle myosin subfragment-1. The inhibitor preferentially binds to the ATPase intermediate with ADP and phosphate bound at the active site, and it slows down phosphate release. Blebbistatin interferes neither with binding of myosin to actin nor with ATP-induced actomyosin dissociation. Instead, it blocks the myosin heads in a products complex with low actin affinity. [3] |
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In vivo | (-)-Blebbistatin ((S)-(-)-Blebbistatin) is a cell-permeable inhibitor for non muscle myosin II ATPase. |
Kinase Assay: |
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Data from [Data independently produced by , , J Leukoc Biol, 2016, 143-53]
Data from [Data independently produced by , , Biomed Res Int, 2018, 2018:2174824]
Data from [Data independently produced by , , J Vasc Res, 2016, 53(3-4):206-215]
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BMP suppresses Wnt signaling via the Bcl11b-regulated NuRD complex to maintain intestinal stem cells [ EMBO J, 2024, ] | PubMed: 39433900 |
Methylcellulose improves dissociation quality of adult human primary cardiomyocytes [ Heliyon, 2024, 10(11):e31653] | PubMed: 38841456 |
Protocol for the dissociation of adult human primary cardiomyocytes using a methylcellulose-supplemented digestion solution [ STAR Protoc, 2024, 5(4):103379] | PubMed: 39412996 |
Cooperative sensing of mitochondrial DNA by ZBP1 and cGAS promotes cardiotoxicity [ Cell, 2023, 186(14):3013-3032.e22] | PubMed: 37352855 |
Cooperative sensing of mitochondrial DNA by ZBP1 and cGAS promotes cardiotoxicity [ Cell, 2023, 186(14):3013-3032.e22] | PubMed: 37352855 |
Cooperative sensing of mitochondrial DNA by ZBP1 and cGAS promotes cardiotoxicity [ Cell, 2023, 186(14):3013-3032.e22] | PubMed: 37352855 |
Hydrogels with tunable mechanical plasticity regulate endothelial cell outgrowth in vasculogenesis and angiogenesis [ Nat Commun, 2023, 14(1):8307] | PubMed: 38097553 |
Drug specificity and affinity are encoded in the probability of cryptic pocket opening in myosin motor domains [ Elife, 2023, 12e83602] | PubMed: 36705568 |
Extracellular flux analysis in intact cardiac tissue slices - a novel tool to investigate cardiac substrate metabolism in mouse myocardium [ Acta Physiol (Oxf), 2023, e14004.] | PubMed: 37227741 |
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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.
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