bisBenzimide H 33258 Trihydrochloride

Catalog No.E2910 Batch:E291001

Technical Data

Formula	C₂₅H₂₄N₆O.3ClH
Molecular Weight	533.88	CAS No.	23491-45-4
Solubility (25°C)*	In vitro	DMSO	31 mg/mL (58.06 mM)
		Water	23 mg/mL (43.08 mM)
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	bisBenzimide H 33258 Trihydrochloride(Hoechst 33258 trihydrochloride; H 33258 trihydrochloride) is a marker dye in Hoechst series. Hoechst is A live nuclear marker dye. It binds to the grooves in the DNA double strand, which tends to be A/ T-rich DNA strand. Although it binds to all nucleic acids, the A/ T-rich double strand DNA significantly enhances fluorescence intensity. Therefore, Hoechst dye can be used for living cell labeling. The fluorescence intensity of Hoechst dye increases with the increase of pH of solution. It exhibits anti-tumor activity IC50 of 51.31±4.56 μM in HeLa cells.

Description

bisBenzimide H 33258 Trihydrochloride(Hoechst 33258 trihydrochloride; H 33258 trihydrochloride) is a marker dye in Hoechst series. Hoechst is A live nuclear marker dye. It binds to the grooves in the DNA double strand, which tends to be A/ T-rich DNA strand. Although it binds to all nucleic acids, the A/ T-rich double strand DNA significantly enhances fluorescence intensity. Therefore, Hoechst dye can be used for living cell labeling. The fluorescence intensity of Hoechst dye increases with the increase of pH of solution. It exhibits anti-tumor activity IC50 of 51.31±4.56 μM in HeLa cells.

Protocol (from reference)

References

[1] Wang XJ, et al. Bioorg Med Chem Lett. 2012 Oct 1;22(19):6297-300.

RETURN POLICY
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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.