BI6015

Catalog No.E0401 Batch:E040103

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Technical Data

Formula

C15H13N3O4S
Molecular Weight 331.35 CAS No. 93987-29-2
Solubility (25°C)* In vitro DMSO 33 mg/mL (99.59 mM)
Water Insoluble
Ethanol Insoluble
In vivo (Add solvents to the product individually and in order)
Clear solution
5% DMSO 95% Corn oil
0.8mg/ml Taking the 1 mL working solution as an example, add 50 μL of 16 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results. 
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O
1.65mg/ml Taking the 1 mL working solution as an example, add 50 μL of 33 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description BI6015, a hepatocyte nuclear factor 4α (HNF4α) antagonist, induces loss of HNF4α expression and hepatic steatosis in vivo. BI6015 is cytotoxic to human hepatocellular carcinoma (HCC).
In vitro

BI-6015 is a hepatocyte nuclear factor 4α (HNF4α) antagonist, which represses insulin promoter activity through HNF4α antagonism.[1]
In vivo

BI-6015 induces loss of HNF4α expression and hepatic steatosis in mice, also induces apoptosis in a human hepatocellular carcinoma mouse model.[1]

Protocol (from reference)

Cell Assay:

[1]
  • Cell lines

    T6PNE, MIN6 and HepG2 cells

  • Concentrations

    1.25, 2.5, 5, 10, 20 μM

  • Incubation Time

    5 or 48 h

  • Method

    T6PNE, MIN6, and HepG2 cells are cultured for 5 or 48 h in the presence of BI6015. HNF4a mRNA levels are normalized to 18S rRNA or GAPDH to control for nonspecific compound effects.
Animal Study:

[1]
  • Animal Models

    mice, human hepatocellular carcinoma mouse model

  • Dosages

    10-30 mg/kg

  • Administration

    i.p.

Selleck's BI6015 has been cited by 1 publication

HNF4α ubiquitination mediated by Peli1 impairs FAO and accelerates pressure overload-induced myocardial hypertrophy [ Cell Death Dis, 2024, 15(2):135] PubMed: 38346961

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.