Bevacizumab (anti-VEGF)

Catalog No.A2006 Batch: A200602

Technical Data

CAS No.	216974-75-3
Formulation	PBS buffer, pH 7.2
Isotype	Human IgG1
Source	CHO cells
Storage (From the date of receipt)	Store the undiluted solution at 4°C in the dark to avoid freeze-thaw cycles
Purity	99.4%
Protein concentration	5mg/ml
Endotoxin Level	≤1 EU/mg

Biological Activity

Description

Bevacizumab (anti-VEGF, Avastin) is a humanized anti-VEGF monoclonal antibody which binds to and neutralizes all human VEGF-A isoforms and bioactive proteolytic fragments, MW:149 KD.

Targets

VEGFR2 ^[4]

VEGF-A ^[4]

VEGF ^[1]

In vitro

Blocking VEGF with bevacizumab reduces the proangiogenic effect of affected fibroblasts in both the matrigel and direct coculture angiogenesis models.^[1]

In vivo

The combinational treatment of AAV9-LECT2-shRNA and bevacizumab significantly improves the therapeutic effects on liver fibrosis.^[2]

Protocol (Only for Reference)

Cell Assay:	Matrigel gels were created using low GF Matrigel (protein concentration > 9.5 mg/ml) in a 48-well plate. It was gelled at 37 ℃ overnight and then rinsed twice with PBS. endothelial cells (ECs) were added in 250 μl endothelial basal medium with no supplement and allowed to adhere for 1 hour. An additional 250 μl of 48 h conditioned medium from unaffected or affected fibroblasts or nonconditioned medium with or without 20 ng/ml VEGF and with or without 250 μl/ml bevacizumab in 5% FBS + 1% penicillin/streptomycin in DMEM was added, and ECs were incubated for 14 h before being fixed and stained for CD31. ECs network formation was also tracked by live-cell imaging at 2, 4, 8, 16, and 24 h.
Animal Study:	Animal Models: Male C57BL/6J mice of CCl4-induced fibrosis model Dosages: 2 mg/kg Administration: i.p. Reference: https://pubmed.ncbi.nlm.nih.gov/34940991/

Cell Assay:

Matrigel gels were created using low GF Matrigel (protein concentration > 9.5 mg/ml) in a 48-well plate. It was gelled at 37 ℃ overnight and then rinsed twice with PBS. endothelial cells (ECs) were added in 250 μl endothelial basal medium with no supplement and allowed to adhere for 1 hour. An additional 250 μl of 48 h conditioned medium from unaffected or affected fibroblasts or nonconditioned medium with or without 20 ng/ml VEGF and with or without 250 μl/ml bevacizumab in 5% FBS + 1% penicillin/streptomycin in DMEM was added, and ECs were incubated for 14 h before being fixed and stained for CD31. ECs network formation was also tracked by live-cell imaging at 2, 4, 8, 16, and 24 h.

Animal Study:

Animal Models: Male C57BL/6J mice of CCl4-induced fibrosis model
Dosages: 2 mg/kg
Administration: i.p.
Reference: https://pubmed.ncbi.nlm.nih.gov/34940991/

References	[1] Hurley-Novatny AC, et al. J Invest Dermatol. 2022 Feb 19:S0022-202X(22)00125-7. [2] Lin Y, et al. Hepatology. 2021 Dec 23.

References

Customer Product Validation

Data from [Data independently produced by , , Cancer Lett, 2018, 439:78-90]

VEGFA expression in the indicated cells treated with or without bevacizumab or transfected with VEGFA or shVEGFA.

Selleck's Bevacizumab (anti-VEGF) has been cited by 49 publications

Tumor Explants Elucidate a Cascade of Paracrine SHH, WNT, and VEGF Signals Driving Pancreatic Cancer Angiosuppression [ Cancer Discov, 2024, 14(2):348-361]	PubMed: 37966260
Leukemic stem cells activate lineage inappropriate signalling pathways to promote their growth [ Nat Commun, 2024, 15(1):1359]	PubMed: 38355578
Engineered therapeutic antibodies with mannose 6-phosphate analogues as a tool to degrade extracellular proteins [ Front Immunol, 2024, 15:1273280]	PubMed: 38533506
Bevacizumab increases the sensitivity of olaparib to homologous recombination-proficient ovarian cancer by suppressing CRY1 via PI3K/AKT pathway [ Front Oncol, 2024, 14:1302850]	PubMed: 38420012
Interferon-regulatory factor-1 boosts bevacizumab cardiotoxicity by the vascular endothelial growth factor A/14-3-3γ axis [ ESC Heart Fail, 2024, 11(2):986-1000]	PubMed: 38234115
Interferon-regulatory factor-1 boosts bevacizumab cardiotoxicity by the vascular endothelial growth factor A/14-3-3γ axis [ ESC Heart Fail, 2024, 10.1002/ehf2.14640]	PubMed: 38234115
Tumor Explants Elucidate a Cascade of Paracrine SHH, WNT, and VEGF Signals Driving Pancreatic Cancer Angiosuppression [ Cancer Discov, 2024, 14(2):348-361]	PubMed: 37966260
EDIL3 as an Angiogenic Target of Immune Exclusion Following Checkpoint Blockade [ Cancer Immunol Res, 2023, 11(11):1493-1507]	PubMed: 37728484
L1CAM promotes vasculogenic mimicry formation by miR-143-3p-induced expression of hexokinase 2 in glioma [ Mol Oncol, 2023, none]	PubMed: 36708044
Vascularized Immunocompetent Patient-Derived Tumor Model to Predict Treatment Response for Lung Cancer Patients [ iScience, 2023, Volume 26, Issue 10]	PubMed: none

FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS.

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