β-Nicotinamide Mononucleotide

Catalog No.S5259 Batch:S525908

Technical Data

Formula	C₁₁H₁₅N₂O₈P
Molecular Weight	334.22	CAS No.	1094-61-7
Solubility (25°C)*	In vitro	Water	67 mg/mL (200.46 mM)
		DMSO	Insoluble
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description	Nicotinamide mononucleotide (β-NM, NMN), one of the key precursors of NAD+ and products of the NAMPT reaction , is converted to NAD+ by nicotinamide mononucleotide adenylyltransferase.
In vitro	Nicotinamide mononucleotide, the NAD+ precursor, rescues the NAD+/NADH ratio and exerts anti-senescent effects by activating SIRT1.^[1]
In vivo	NMN reduces the loss of cochlear hair cells caused by cisplatin and improves hearing in C57BL/6 mice, by supplementation of NAD+ through de novo and salvage synthesis pathways.^[2]

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines mouse AT2 cells strain MLE-12 Concentrations 500 μM Incubation Time -- Method The mouse AT2 cells strain MLE-12 are cultured in DMEM with 10% FBS at 37 ℃ in a humidified atmosphere containing 5% CO₂. Cells are treated with rapamycin (10 nM), bafilomycin (5 nM), 3 MA (3 mM), SRT1720 (4 μM), Ex527 (10 μM), NMN (500 μM), Olaparib (40 μM) and mitoquinone (50 nM). β-galactosidase staining is performed based on the manufacture s instructions. Relative protein expression levels are measured by Western blot. The level of NAD+/NADH is quantified by an NAD+/NADH detection kit.
Animal Study:^{^[2]}	Animal Models C57BL/6 mice Dosages 100 mg/kg Administration i.g., i.p.

Cell Assay:^{^[1]}

Cell lines
mouse AT2 cells strain MLE-12
Concentrations
500 μM
Incubation Time
--
Method
The mouse AT2 cells strain MLE-12 are cultured in DMEM with 10% FBS at 37 ℃ in a humidified atmosphere containing 5% CO₂. Cells are treated with rapamycin (10 nM), bafilomycin (5 nM), 3 MA (3 mM), SRT1720 (4 μM), Ex527 (10 μM), NMN (500 μM), Olaparib (40 μM) and mitoquinone (50 nM). β-galactosidase staining is performed based on the manufacture s instructions. Relative protein expression levels are measured by Western blot. The level of NAD+/NADH is quantified by an NAD+/NADH detection kit.

Animal Study:^{^[2]}

Animal Models
C57BL/6 mice
Dosages
100 mg/kg
Administration
i.g., i.p.

References

Selleck's β-Nicotinamide Mononucleotide has been cited by 22 publications

SIRT1 stabilizes β-TrCP1 to inhibit Snail1 expression in maintaining intestinal epithelial integrity to alleviate colitis [ Cell Mol Gastroenterol Hepatol, 2024, S2352-345X(24)00108-5]	PubMed: 38729522
NAD+ regulates nucleotide metabolism and genomic DNA replication [ Nat Cell Biol, 2023, 10.1038/s41556-023-01280-z]	PubMed: 37957325
ABCA7 deficiency causes neuronal dysregulation by altering mitochondrial lipid metabolism [ Mol Psychiatry, 2023, 10.1038/s41380-023-02372-w]	PubMed: 38135757
Temperature-sensitive hydrogel dressing loaded with nicotinamide mononucleotide accelerating wound healing in diabetic mice [ Biomed Pharmacother, 2023, 10.1016/j.biopha.2023.115431]	PubMed: 37688988
β-nicotinamide mononucleotide rescues the quality of aged oocyte and improves subsequent embryo development in pigs [ PLoS One, 2023, 18(10):e0291640]	PubMed: 37796824
DePARylation is critical for S phase progression and cell survival [ bioRxiv, 2023, 2023.07.31.551317]	PubMed: 37577639
DePARylation is critical for S phase progression and cell survival [ bioRxiv, 2023, 2023.07.31.551317]	PubMed: 37577639
Ezh2 competes with p53 to license lncRNA Neat1 transcription for inflammasome activation [ Cell Death Differ, 2022, 10.1038/s41418-022-00992-3]	PubMed: 35568718
Administration of nicotinamide mononucleotide improves oocyte quality of obese mice [ Cell Prolif, 2022, e13303]	PubMed: 35811338
DNA damage contributes to age-associated differences in SARS-CoV-2 infection [ Aging Cell, 2022, 21(12):e13729]	PubMed: 36254583

RETURN POLICY
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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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