Benzoylaconitine

Catalog No.S0944 Batch:S094401

Technical Data

Formula	C₃₂H₄₅NO₁₀
Molecular Weight	603.70	CAS No.	466-24-0
Solubility (25°C)*	In vitro	DMSO	100 mg/mL (165.64 mM)


* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	Benzoylaconine (Isaconitine, Pikraconitin) is an alkaloid derived from the Chinese traditional medicine Radix Aconiti Lateralis Preparata (Fuzi). Benzoylaconitine inhibits production of IL-6 and IL-8 via MAPK, Akt, NF-κB signaling in IL-1β-induced human synovial cells.
In vitro	Benzoylaconitine is a lower toxic monoester type alkaloid considered as the pharmacodynamic constituent in Aconitum species, which exerts an anti-inflammatory effect dependent on MAPK, Akt and nuclear factor-κB (NF-κB) pathways in human synovial cells stimulated with IL-1β.^[1]
In vivo	Benzoylaconitine shows efficient in vivo anti-inflammatory effect with high ear (69.8 %) and paw (87.1 %) swelling suppressing rate.^[3]

Protocol (from reference)

Cell Assay:^{^[2]}	Cell lines IL-1β-stimulated human synovial SW982 cells Concentrations 5, 10 µM Incubation Time 30 min Method The SW982 cells are pretreated with various doses (0, 5 and 10 μM) of BAC for 30 min, followed by IL-1β stimulation (10 ng/mL) for 12 h. The levels of IL-6 and IL-8 in supernatants of cell cultures are determined by ELISA kits according to the manufacturer's recommendations.

Cell Assay:^{^[2]}

Cell lines
IL-1β-stimulated human synovial SW982 cells
Concentrations
5, 10 µM
Incubation Time
30 min
Method
The SW982 cells are pretreated with various doses (0, 5 and 10 μM) of BAC for 30 min, followed by IL-1β stimulation (10 ng/mL) for 12 h. The levels of IL-6 and IL-8 in supernatants of cell cultures are determined by ELISA kits according to the manufacturer's recommendations.

References

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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