BCH001

Catalog No.S8977 Batch:S897701

Technical Data

Formula	C₂₀H₁₅F₃N₂O₅
Molecular Weight	420.34	CAS No.	384859-58-9
Solubility (25°C)*	In vitro	DMSO	84 mg/mL (199.83 mM)
		Ethanol	5 mg/mL (11.89 mM)
		Water	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description

BCH001 is a specific PAPD5 inhibitor that restores telomerase activity and telomere length in dyskeratosis congenita (DC) patient induced pluripotent stem cells. PAPD5 is a noncanonical poly(A) polymerase with an unusual RNA-binding motif.

Targets

PAPD5 ^[1]

In vitro

BCH001 is a specific PAPD5 inhibitor that restores telomerase activity and telomere length in DC patient induced pluripotent stem cells.^[1]

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines CD34+ HSPCs, iPSCs and fibroblasts Concentrations 1 μM, 5 μM, 100 μM Incubation Time 72 h, 5 days, 14 days Method Equal numbers of DC patient iPSCs are plated and 24 hr later treated with 1 μM BCH001 versus DMSO as vehicle control. 72 hr post-treatment cells are harvested and fixed using 70% ethanol at 4 ℃ overnight. For CD34+ HSPCs, 1000 cells are plated in methylcellulose medium containing human cytokines and inhibitors BCH001, RG7834 or vehicle (DMSO). After 14 days of culture at 37℃, 5% CO2, the progenitor colonies are counted manually under the microscope, with categories defined by colony morphology.

Cell Assay:^{^[1]}

Cell lines
CD34+ HSPCs, iPSCs and fibroblasts
Concentrations
1 μM, 5 μM, 100 μM
Incubation Time
72 h, 5 days, 14 days
Method
Equal numbers of DC patient iPSCs are plated and 24 hr later treated with 1 μM BCH001 versus DMSO as vehicle control. 72 hr post-treatment cells are harvested and fixed using 70% ethanol at 4 ℃ overnight.
For CD34+ HSPCs, 1000 cells are plated in methylcellulose medium containing human cytokines and inhibitors BCH001, RG7834 or vehicle (DMSO). After 14 days of culture at 37℃, 5% CO2, the progenitor colonies are counted manually under the microscope, with categories defined by colony morphology.

References

[1] Neha Nagpal, et al. Cell Stem Cell. 2020 Jun 4;26(6):896-909.e8.

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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