BC1618

Catalog No.S9860 Batch:S986001

Technical Data

Formula	C₂₄H₂₄F₃NO₂
Molecular Weight	415.45	CAS No.	2222094-18-8
Solubility (25°C)*	In vitro	DMSO	83 mg/mL (199.78 mM)
		Ethanol	83 mg/mL (199.78 mM)
		Water	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description

BC1618 is an orally active Fbxo48 inhibitor that stimulates adenosine monophosphate (AMP)-activated protein kinase (Ampk)-dependent signaling. BC1618 promotes mitochondrial fission, facilitates autophagy and improves hepatic insulin sensitivity.

Targets

Fbxo48 ^[1]

In vitro

BC1618 interrupts Fbxo48/pAmpkα interaction. BC1618 facilitates mitochondrial fission and autophagy.^[1]

In vivo

Consistent with augmenting Ampk activity, BC1618 promotes mitochondrial fission, facilitates autophagy and improves hepatic insulin sensitivity in high-fat-diet-induced obese mice.^[1]

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines 293T cells Concentrations 3 μM Incubation Time 30 min Method 293T cells transfected with Fbxo48-V5 or Fbxo30-V5 are treated with control (DMSO) or BC1618 (3 μM) for 30 min. Cells are then washed and equally fractioned into 10 aliquots and subjected to a range of increasing temperatures to promote protein denaturation. After a high-speed spin to precipitate heat-denatured proteins, the soluble protein fraction from each temperature is then immunoblotted for Fbxo48 or Fbxo30.
Animal Study:^{^[1]}	Animal Models C57BL/6 mice Dosages 15 mg/kg, 20 mg/kg, 30 mg/kg Administration Oral gavage

Cell Assay:^{^[1]}

Cell lines
293T cells
Concentrations
3 μM
Incubation Time
30 min
Method
293T cells transfected with Fbxo48-V5 or Fbxo30-V5 are treated with control (DMSO) or BC1618 (3 μM) for 30 min. Cells are then washed and equally fractioned into 10 aliquots and subjected to a range of increasing temperatures to promote protein denaturation. After a high-speed spin to precipitate heat-denatured proteins, the soluble protein fraction from each temperature is then immunoblotted for Fbxo48 or Fbxo30.

Animal Study:^{^[1]}

Animal Models
C57BL/6 mice
Dosages
15 mg/kg, 20 mg/kg, 30 mg/kg
Administration
Oral gavage

References

[1] Yuan Liu, et al. Nat Chem Biol. 2021 Mar;17(3):298-306.

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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