BAY-885

Catalog No.S8896 Batch:S889601

Technical Data

Formula	C₂₅H₂₈F₃N₇O₂
Molecular Weight	515.53	CAS No.	2307249-33-6
Solubility (25°C)*	In vitro	DMSO	30 mg/mL (58.19 mM)
		Ethanol	8 mg/mL (15.51 mM)
		Water	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description

BAY-885 is a highly potent and selective ERK5 inhibitor.

Targets

ERK5 ^[1]

In vitro

BAY-885 shows potent ERK5 kinase and transcriptional inhibition in the SN12C-MEF2 reporter cell line (IC50 = 115 nM/IC90 = 691 nM) and has no effects on a reporter control cell line with constitutive luciferase expression (SN12C-CMV-luc, IC50 > 30 μM), thereby ruling out potential effects as a general inhibitor of transcription or translation.^[1]

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines BT-474, SK-BR-3, SNU-449, NCIH460, SN12C, MFM-223 Concentrations 0.07 nM - 20 μM Incubation Time 16 h Method On day 1, the cells are seeded in 384-well white plates at a density of 10,000 cells per well in 20 μL of culture medium. On day 2, the test compounds including BAY-885 are added in serial dilutions using the HP D300 Digital Dispenser and incubated at 37 °C for 16 h.

Cell Assay:^{^[1]}

Cell lines
BT-474, SK-BR-3, SNU-449, NCIH460, SN12C, MFM-223
Concentrations
0.07 nM - 20 μM
Incubation Time
16 h
Method
On day 1, the cells are seeded in 384-well white plates at a density of 10,000 cells per well in 20 μL of culture medium. On day 2, the test compounds including BAY-885 are added in serial dilutions using the HP D300 Digital Dispenser and incubated at 37 °C for 16 h.

References

[1] Duy Nguyen, et al. J Med Chem. 2019 Jan 24;62(2):928-940.

Selleck's BAY-885 has been cited by 1 publication

BAY-885, a mitogen-activated protein kinase kinase 5 inhibitor, induces apoptosis by regulating the endoplasmic reticulum stress/Mcl-1/Bim pathway in breast cancer cells [ Bioengineered, 2022, 13(5):12888-12898]	PubMed: 35609325

BAY-885, a mitogen-activated protein kinase kinase 5 inhibitor, induces apoptosis by regulating the endoplasmic reticulum stress/Mcl-1/Bim pathway in breast cancer cells [ Bioengineered, 2022, 13(5):12888-12898]

PubMed: 35609325

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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