Atezolizumab (anti-PD-L1)

Catalog No.A2004        Batch: A200409

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Technical Data

CAS No. 1380723-44-3
Formulation 100 mM Pro-Ac, 20mM Arg, pH5.0
Isotype Human IgG1
Source CHO cells
Storage
(From the date of receipt)		 Store the undiluted solution at 4°C in the dark to avoid freeze-thaw cycles
Purity 99%
Protein concentration 7.2mg/ml
Endotoxin Level ＜1EU/mg

Biological Activity

Description Atezolizumab (anti-PD-L1) is a fully humanized, IgG1 monoclonal antibody that blocks the interaction of PD-L1 with both PD-1 and B7.1, but not the interaction of PD-L2 with PD-1. MW : 145 KD.
Targets
hPD-L1 [2]
(Cell-free assay)
0.4 nM(Kd)
In vitro

Treatment with single agent atezolizumab exerted a moderate anti-proliferative effect with ~ 30% cell death across all models.[1]
In vivo

when dual drug–loaded, core-shell nanoparticles (DLTPT) is used in combination with anti PD-L1, the tumor growth is inhibited, which induced immune response against tumor metastasis.[2]

Protocol (Only for Reference)

Cell Assay:

  • A MTT cell proliferation assays in human spheroids (A) or EPCAM+/CD45- cells (b), untreated or treated with selumetinib, atezolizumab, avelumab, or their combinations.
Animal Study:

  • Animal Models: 4T1 tumor–bearing BALB/c mice
    Dosages: 2.5 or 5 mg/kg
    Administration: i.v.
    Reference:[2]

Customer Product Validation

Selleck's Atezolizumab (anti-PD-L1) has been cited by 68 publications

PD-L1 and IFN-γ modulate Non-Small Cell Lung Cancer (NSCLC) cell plasticity associated to immune checkpoint inhibitor (ICI)-mediated hyperprogressive disease (HPD) [ J Transl Med, 2025, 23(1):2] PubMed: 39748404
PD-L1 monoclonal antibody alleviated MI injury of left ventricular function via modulating CD47/SHP2/SIRPα/SYK/FcγR signalings in tumor associated macrophages [ Sci Rep, 2025, 15(1):2303] PubMed: 39824849
In situ editing of tumour cell membranes induces aggregation and capture of PD-L1 membrane proteins for enhanced cancer immunotherapy [ Nat Commun, 2024, 15(1):9723] PubMed: 39521768
Miltefosine reinvigorates exhausted T cells by targeting their bioenergetic state [ Cell Rep Med, 2024, 5(12):101869] PubMed: 39657666
Favorable impact of PD1/PD-L1 antagonists on bone remodeling: an exploratory prospective clinical study and ex vivo validation [ J Immunother Cancer, 2024, 12(5)e008669] PubMed: 38702145
Respiratory complex I regulates dendritic cell maturation in explant model of human tumor immune microenvironment [ J Immunother Cancer, 2024, 12(4)e008053] PubMed: 38604809
KEAP1 promotes anti-tumor immunity by inhibiting PD-L1 expression in NSCLC [ Cell Death Dis, 2024, 15(2):175] PubMed: 38413563
ALK5/VEGFR2 dual inhibitor TU2218 alone or in combination with immune checkpoint inhibitors enhances immune-mediated antitumor effects [ Cancer Immunol Immunother, 2024, 73(10):190] PubMed: 39105882
[ Int Immunopharmacol, 2024, ] PubMed: 38442580
Polymer-based antibody mimetics (iBodies) target human PD-L1 and function as a potent immune checkpoint blocker [ J Biol Chem, 2024, S0021-9258(24)01826-X] PubMed: 38685532

FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS.

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