Aprotinin

Catalog No.S7377 Batch:S737705

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Technical Data

Formula

C284H432N84O79S7
Molecular Weight 6511.51 CAS No. 9087-70-1
Solubility (25°C)* In vitro Water 100 mg/mL (15.35 mM)
 
 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description Aprotinin is a small protein serine protease inhibitor (Kd=0.06 pM for bovine β-trypsin), used to reduce perioperative blood loss and transfusion.
Targets
Thrombin [1]
(Cell-free assay)		 Trypsin [1]
(Cell-free assay)		 kallikrein [1]
(Cell-free assay)		 chymotrypsin [1]
(Cell-free assay)		 trypsinogen [1]
(Cell-free assay)
0.06 pM(Kd) 0.8 nM(Kd) 9.5 nM(Kd) 2 μM(Kd)
In vitro Aprotinin is an antifibrinolytic molecule that inhibits trypsin and related proteolytic enzymes. In cell biology, aprotinin is used as an enzyme inhibitor to prevent protein degradation during lysis or homogenization of cells and tissues. In the presence of aprotinin, the fibrinolytic activity is inhibited concentration dependently and the coagulation time is prolonged. Aprotinin is an effective inhibitor of the contact (intrinsic) coagulation pathway in vitro[2].
In vivo Aprotinin inhibits clot lysis in vitro as well as rat-tail bleeding time in vivo and prolongs coagulation time in human plasma. In a rat arteriovenous shunt model, aprotinin reduces thrombus weight [2].

Protocol (from reference)

Cell Assay:

[3]
  • Cell lines

    mouse G8-1 and C2C12 skeletal muscle myoblasts

  • Concentrations

    2 TIU/ml

  • Incubation Time

    12hrs

  • Method

    Mouse G8-1 myoblasts are plated DMEM + 20% FBS (maintenance medium), in which they remain undifferentiated. When cells reach approximately 40-50% confluence, different protease inhibitors are added to the culture media and cells are incubated overnight. Cells are then switched to differentiation-promoting media (DMEM + 10% horse serum ± protease inhibitor) and incubated for 7 days.
Animal Study:

[2]
  • Animal Models

    Male Wistar rats

  • Dosages

    1.5 mg/kg and 3 mg/kg/h, 3 mg/kg and 6 mg/kg/h up to 5 mg/kg and 10 mg/kg/h

  • Administration

    bolus injection followed by a maintenance infusion(i.p.)

Selleck's Aprotinin has been cited by 2 publications

SARS-CoV-2 requires cholesterol for viral entry and pathological syncytia formation [ Elife, 2021, 10e65962] PubMed: 33890572
Acetylation targets HSD17B4 for degradation via the CMA pathway in response to estrone. [ Autophagy, 2017, 13(3):538-553] PubMed: 28296597

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.