Angiotensin (1-7)

Catalog No.S9820 Batch:S982001

Technical Data

Formula	C₄₁H₆₂N₁₂O₁₁
Molecular Weight	899.00	CAS No.	51833-78-4
Solubility (25°C)*	In vitro	Water	50 mg/mL (55.61 mM)
		DMSO	Insoluble
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description

Angiotensin (1-7) (Ang-(1-7), Angiotensin fragment 1-7) is a bioactive component of the renin-angiotensin system that is formed endogenously from either Ang I or Ang II. Angiotensin (1-7) is a canine ACE inhibitor with an IC50 of 0.65 μM and inhibits the activity mediated by myostatin through Mas receptor.

Targets

Mas receptor ^[1]	ACE ^[1]
	0.65 μM

In vitro

Angiotensin (1-7) prevents the decrease of the myotube diameter and MHC Levels Induced by myostatin in C₂C₁₂ cells. Angiotensin (1-7) decreases the myostatin-dependent increment of E3 ubiquitin ligases and ROS, the NF-κB signaling induced by myostatin, the myostatin-dependent activity through the mas receptor and Akt/PKB activity in C₂C₁₂ myotubes.^[1]

In vivo

Daily Angiotensin (1-7) administration significantly reduces colitis severity observable at both gross and histological levels in DSS treated mice. The anti-inflammatory effects of Angiotensin (1-7) treatment are associated with a reduction in the phosphorylated forms of p38 MAPK, ERK1/2 and Akt post DSS induction, the anti-inflammatory properties of Angiotensin (1-7) are in part mediated through reduction of Ang II levels.^[3]

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines C₂C₁₂ cells Concentrations 10 nM Incubation Time 30 min, 60 min Method The C₂C₁₂ cells are differentiated until day 5 by changing DMEM–FSB to DMEM supplemented with horse serum. The myotubes are incubated with 1 µg/ml of human recombinant myostatin and/or 10 nM of Angiotensin (1-7). The myotubes are treated with 10 µM of MK2206 for Akt/PKB inhibition, or 10 µM of A779 antagonist for Mas receptor inhibition. In brief, the procedure is as follows: differentiated cells are pre-treated with Angiotensin (1-7) for 30 or 60 min, depending on the experiments, and then treated with myostatin.
Animal Study:^{^[3]}	Animal Models DSS treated mice Dosages 0.01 mg/kg, 0.06 mg/kg, 0.1 mg/kg, 0.3 mg/kg, 1 mg/kg Administration i.p.

Cell Assay:^{^[1]}

Cell lines
C₂C₁₂ cells
Concentrations
10 nM
Incubation Time
30 min, 60 min
Method
The C₂C₁₂ cells are differentiated until day 5 by changing DMEM–FSB to DMEM supplemented with horse serum. The myotubes are incubated with 1 µg/ml of human recombinant myostatin and/or 10 nM of Angiotensin (1-7). The myotubes are treated with 10 µM of MK2206 for Akt/PKB inhibition, or 10 µM of A779 antagonist for Mas receptor inhibition. In brief, the procedure is as follows: differentiated cells are pre-treated with Angiotensin (1-7) for 30 or 60 min, depending on the experiments, and then treated with myostatin.

Animal Study:^{^[3]}

Animal Models
DSS treated mice
Dosages
0.01 mg/kg, 0.06 mg/kg, 0.1 mg/kg, 0.3 mg/kg, 1 mg/kg
Administration
i.p.

References

Selleck's Angiotensin (1-7) has been cited by 1 publication

ACE2 negatively regulates the Warburg effect and suppresses hepatocellular carcinoma progression via reducing ROS-HIF1α activity [ Int J Biol Sci, 2023, 19(8):2613-2629]	PubMed: 37215979

ACE2 negatively regulates the Warburg effect and suppresses hepatocellular carcinoma progression via reducing ROS-HIF1α activity [ Int J Biol Sci, 2023, 19(8):2613-2629]

PubMed: 37215979

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