Anacardic Acid

Catalog No.S7582 Batch:S758204

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Technical Data

Formula

C22H36O3
Molecular Weight 348.52 CAS No. 16611-84-0
Solubility (25°C)* In vitro DMSO 70 mg/mL (200.84 mM)
Ethanol 70 mg/mL (200.84 mM)
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description Anacardic Acid (6-pentadecylsalicylic Acid) is a potent inhibitor of p300 and p300/CBP-associated factor histone acetyltranferases, which also has antibacterial activity, antimicrobial activity, prostaglandin synthase inhibition, and tyrosinase and lipoxygenase inhibition.
Targets
Tip60 [2]
(Cell-free assay)		 PCAF [1]
(Cell-free assay)		 p300/CBP [1]
(Cell-free assay)
5 μM 8.5 μM
In vitro Anacardic acid is found to be a common inhibitor of several clinically targeted enzymes such as NFkB kinase, histone acetyltransferase (HATs), lipoxygenase (LOX-1), xanthine oxidase, tyrosinase and ureases[4]. Anacardic acid is also an efficient inhibitor of Tip60 in vitro.It inhibits the Tip60-dependent acetylation and activation of the ATM protein kinase in HeLa cells, and sensitizes the cells to the cytotoxic effects of radiation[2]. Anacardic acid can effectively prevent cell damage induced by H2O2 because this can be converted to ROS, hydroxyl radicals, in the presence of metal ions[4].
In vivo Anacardic acid attenuates over-expression of Gata4, α-MHC and cTnT in fetal mouse hearts exposed to ethanol. Anacardic acid inhibits binding of P300, PCAF to the promoter of Gata4[3].

Protocol (from reference)

Kinase Assay:[3]
  • HAT and HDAC Activity Assays

    After homogenization of cardiac tissues, nucleoproteins are extracted using a Nuclear Extract Kit according to the manufacturer's instructions. HAT and HDAC activities of the nuclear protein extracts are determined using a colorimetric assay included in the HAT and HDAC assay kits.
Cell Assay:[2]
  • Cell lines

    HeLa and 293T cells, SQ20B and SCC35 squamous cell carcinoma cell lines

  • Concentrations

    0-100 μM

  • Incubation Time

    40 min

  • Method

    HeLa and 293T cells are grown in Dulbecco’s modified Eagle’s medium/10% fetal calf serum. SQ20B and SCC35 squamous cell carcinoma cell lines are cultured in modified eagles medium/10% fetal calf serum.For anacardic acid (EMD Biosciences, CA) exposure, cells are preincubated with anacardic acid for 40 min, irradiated, then allowed to recover for 4 h. Cells are then switched to fresh media and allowed to grow for 10 days.
Animal Study:[3]
  • Animal Models

    9 to 11 week old Kunming mice

  • Dosages

    0, 1.25, 2.5, 5, 10 mg/kg

  • Administration

    i.p.

Customer Product Validation

Data from [Data independently produced by , , Front Immunol, 2017, 7:696]

Data from [Data independently produced by , , Oncol Rep, 2017, 38(1):229-236]

Selleck's Anacardic Acid has been cited by 22 publications

The mechanics of local niches in the primary tumor regulate breast cancer brain metastasis [ POLYU ELECTRONIC THESES, 2023, viii, 164] PubMed: None
HBV covalently closed circular DNA minichromosomes in distinct epigenetic transcriptional states differ in their vulnerability to damage [ Hepatology, 2021, 10.1002/hep.32245] PubMed: 34779008
HBV covalently closed circular DNA minichromosomes in distinct epigenetic transcriptional states differ in their vulnerability to damage [ Hepatology, 2021, 10.1002/hep.32245] PubMed: 34779008
Caspase-8 Regulates the Antimyeloma Activity of Bortezomib and Lenalidomide [ J Pharmacol Exp Ther, 2021, 379(3):303-309] PubMed: 34588172
Anacardic acid induces IL-33 and promotes remyelination in CNS [ Proc Natl Acad Sci U S A, 2020, 202006566] PubMed: 32817520
Histone Acetyltransferase (HAT) P300/CBP Inhibitors Induce Synthetic Lethality in PTEN-Deficient Colorectal Cancer Cells Through Destabilizing AKT [ Int J Biol Sci, 2020, 25;16(11):1774-1784] PubMed: 32398948
Histone acetyltransferase promotes fluoride toxicity in LS8 cells. [ Chemosphere, 2020, 247:125825] PubMed: 31927229
Identification of Sca-1+Abcg1+ bronchioalveolar epithelial cells as the origin of lung adenocarcinoma in Gprc5a-knockout mouse model through the interaction between lung progenitor AT2 and Lgr5 cells [ Oncogene, 2020, 39(18):3754-3773] PubMed: 32157214
Identification of Sca-1+Abcg1+ bronchioalveolar epithelial cells as the origin of lung adenocarcinoma in Gprc5a-knockout mouse model through the interaction between lung progenitor AT2 and Lgr5 cells. [ Oncogene, 2020, 39(18):3754-3773] PubMed: 32157214
Butyrate alleviates oxidative stress by regulating NRF2 nuclear accumulation and H3K9/14 acetylation via GPR109A in bovine mammary epithelial cells and mammary glands. [ Free Radic Biol Med, 2020, 10.1016/j.freeradbiomed.2020.01.016] PubMed: 31972340

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.