Amprolium HCl

Catalog No.S4144 Batch:S414401

Technical Data

Formula	C₁₄H₁₉N₄.HCl.Cl
Molecular Weight	315.24	CAS No.	137-88-2
Solubility (25°C)*	In vitro	Water	63 mg/mL (199.84 mM)
		DMSO	2 mg/mL (6.34 mM)
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description	Amprolium chloride is a thiamin antagonist, which prevents carbohydrate synthesis by blocking thiamine uptake.
In vivo	Amprolium (APL) is administrated intravenously at dose of 13 mg/kg in healthy chickens, the half-life beta t_1/2, volume of distribution (Vd) and total body clearance (Cl) after intravenous administration are 0.21 hour, 0.12 L/kg and 1.32 L/h.kg, respectively. Amprolium (APL) (13 mg/kg, orally) results in the elimination half-life (t_1/2) of 0.292 hour-0.654 hour which is 1.5-3.2 times longer than after intravenous administration, suggesting the presence of a 'flip-flop' phenomenon in chickens. Amprolium (APL) (13 mg/kg, orally) results in the maximum plasma concentration (C_max) of 13 mg/kg during fasting which is about four times higher than that during nonfasting, and bioavailability during nonfasting is from 2.3% to 2.6%, and 6.4% during fasting. ^[1] Amprolium (0.1%) feeding significantly reduces total thiamin levels in heart, liver, and muscle but not in kidney in Broodfish of Atlantic salmon Salmo salar. ^[2] Amprolium hydrochloride (30 mg/kg, i.v.) results in t1/2 alpha of 0.17 hour, t_1/2 beta of 4.89 hours, the total body clearance of 0.562 mL/kg/min, volume of distribution at steady state of 0.34 L/kg in Hubbard broiler chickens. Amprolium hydrochloride (30 mg/kg, i.v.) results in the peak plasma concentrations (C_max) of 42.9 mg/mL and occurred at 3.67 hours, and systemic bioavailability of 66% in Hubbard broiler chickens. ^[3] Amprolium (125 ppm) shows significantly lower lesion scores than infected non medicated group in broiler chickens infected Eimeria tenella. Amprolium (125 ppm) significantly reduces mortality compared with infected non medicated group in broiler chickens infected Eimeria tenella. ^[4]

Description

Amprolium chloride is a thiamin antagonist, which prevents carbohydrate synthesis by blocking thiamine uptake.

In vivo

Amprolium (APL) is administrated intravenously at dose of 13 mg/kg in healthy chickens, the half-life beta t_1/2, volume of distribution (Vd) and total body clearance (Cl) after intravenous administration are 0.21 hour, 0.12 L/kg and 1.32 L/h.kg, respectively. Amprolium (APL) (13 mg/kg, orally) results in the elimination half-life (t_1/2) of 0.292 hour-0.654 hour which is 1.5-3.2 times longer than after intravenous administration, suggesting the presence of a 'flip-flop' phenomenon in chickens. Amprolium (APL) (13 mg/kg, orally) results in the maximum plasma concentration (C_max) of 13 mg/kg during fasting which is about four times higher than that during nonfasting, and bioavailability during nonfasting is from 2.3% to 2.6%, and 6.4% during fasting. ^[1] Amprolium (0.1%) feeding significantly reduces total thiamin levels in heart, liver, and muscle but not in kidney in Broodfish of Atlantic salmon Salmo salar. ^[2] Amprolium hydrochloride (30 mg/kg, i.v.) results in t1/2 alpha of 0.17 hour, t_1/2 beta of 4.89 hours, the total body clearance of 0.562 mL/kg/min, volume of distribution at steady state of 0.34 L/kg in Hubbard broiler chickens. Amprolium hydrochloride (30 mg/kg, i.v.) results in the peak plasma concentrations (C_max) of 42.9 mg/mL and occurred at 3.67 hours, and systemic bioavailability of 66% in Hubbard broiler chickens. ^[3] Amprolium (125 ppm) shows significantly lower lesion scores than infected non medicated group in broiler chickens infected Eimeria tenella. Amprolium (125 ppm) significantly reduces mortality compared with infected non medicated group in broiler chickens infected Eimeria tenella. ^[4]

Protocol (from reference)

References

[4] ABBAS Rao Z, et al. Pesq. Vet. Bras, 2011, 31(5), 425-429.

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Selleck's Amprolium HCl has been cited by 2 publications

Thiamine-modified metabolic reprogramming of human pluripotent stem cell-derived cardiomyocyte under space microgravity [ Signal Transduct Target Ther, 2024, 9(1):86]	PubMed: 38584163
Maternal vitamin B1 is a determinant for the fate of primordial follicle formation in offspring [ Nat Commun, 2023, 10.1038/s41467-023-43261-8]	PubMed: 37973927

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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