AOA (Aminooxyacetic acid) hemihydrochloride

Catalog No.S4989 Batch:S498901

Technical Data

Formula	C₂H₅NO₃._1/2HCl
Molecular Weight	109.3	CAS No.	2921-14-4
Solubility (25°C)*	In vitro	DMSO	21 mg/mL (192.13 mM)


* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description

AOA (Aminooxyacetic acid) hemihydrochloride is an inhibitor of aminobutyrate aminotransferase involved in amino acid and polyamine metabolism. AOA hemihydrochloride is also a CBS (cystathionine β-synthase) inhibitor.

Targets

GABA-T ^[1]

Malate-aspartate shuttle ^[1]

In vitro

Aminooxyacetic acid (AOAA) is a malate-aspartate shuttle inhibitor, leading to decreased intracellular ATP levels and altering cell cycle and increasing apoptosis and necrosis of C6 glioma cells, without affecting the survival of primary astrocyte cultures. AOAA also decreases the glycolytic rate and the levels of extracellular lactate and pyruvate, without affecting the mitochondrial membrane potential of C6 cells. Aminooxyacetic acid can selectively inhibit the proliferation of breast cancer cells^[1].

In vivo

Aminooxyacetic acid suppresses the growth of breast adenocarcinoma in athymic mice^[1]. AOAA is much less toxic in rats than in mice^[2].

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines C6 glioma cells Concentrations 0.1, 0.25, 0.5, 1, 5 mM Incubation Time 24 h Method C6 glioma cells are plated into 24-well or 12-well cell culture plates at the initial density of 1 × 10⁶ cells/mL in Dulbecco's Modified Eagle's Medium containing 4500 mg/L D-glucose, 584 mg/L L-glutamine, and 1% penicillin and streptomycin, supplemented with 10% fetal bovine serum. The cells are maintained in a 5% CO2 incubator at 37℃. Cell survival is assessed 24 h after AOAA treatment by intracellular LDH assay.
Animal Study:^{^[2]}	Animal Models female Wistar rats Dosages 15 to 100 mg/kg Administration i.p. or s.c.

Cell Assay:^{^[1]}

Cell lines
C6 glioma cells
Concentrations
0.1, 0.25, 0.5, 1, 5 mM
Incubation Time
24 h
Method
C6 glioma cells are plated into 24-well or 12-well cell culture plates at the initial density of 1 × 10⁶ cells/mL in Dulbecco's Modified Eagle's Medium containing 4500 mg/L D-glucose, 584 mg/L L-glutamine, and 1% penicillin and streptomycin, supplemented with 10% fetal bovine serum. The cells are maintained in a 5% CO2 incubator at 37℃. Cell survival is assessed 24 h after AOAA treatment by intracellular LDH assay.

Animal Study:^{^[2]}

Animal Models
female Wistar rats
Dosages
15 to 100 mg/kg
Administration
i.p. or s.c.

References

Selleck's AOA (Aminooxyacetic acid) hemihydrochloride has been cited by 12 publications

NRF2 activation by cysteine as a survival mechanism for triple-negative breast cancer cells [ Oncogene, 2024, 43(22):1701-1713]	PubMed: 38600165
NRF2 activation by cysteine as a survival mechanism for triple-negative breast cancer cells [ Research Square, 2024, 10.21203/rs.3.rs-3849836/v1]	PubMed: none
Dysregulated Gln-Glu-α-ketoglutarate axis impairs maternal decidualization and increases the risk of recurrent spontaneous miscarriage [ Cell Rep Med, 2023, 4(5):101026]	PubMed: 37137303
Metabolic rewiring in keratinocytes by miR-31-5p identifies therapeutic intervention for psoriasis [ EMBO Mol Med, 2023, e15674.]	PubMed: 36855912
The mitochondrial NADH shuttle system is a targetable vulnerability for Group 3 medulloblastoma in a hypoxic microenvironment [ Cell Death Dis, 2023, 14(11):784]	PubMed: 38036520
L-cystathionine protects against oxidative stress and DNA damage induced by oxidized low-density lipoprotein in THP-1-derived macrophages [ Front Pharmacol, 2023, 14:1161542]	PubMed: 37560474
Glutamine Metabolism Is Required for Alveolar Regeneration during Lung Injury [ Biomolecules, 2022, 12(5)728]	PubMed: 35625656
Glutamate dehydrogenase 1 mediated glutaminolysis sustains HCC cells survival under glucose deprivation [ J Cancer, 2022, 13(3):1061-1072]	PubMed: 35154470
Yap-Myc signaling induces pancreatic stellate cell activation through regulating glutaminolysis [ Exp Cell Res, 2022, 411(1):113000]	PubMed: 34958764
Mistimed H 2 S Upregulation, Nrf2 Activation and Antioxidant Proteins Levels in Renal Tubular Epithelial Cells Subjected to Anoxia and Reoxygenation [ Biomed Rep, 2020, 13(2):3]	PubMed: 32509306

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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