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Technical Data
| Formula | C6H8ClN7O.HCl.2H2O |
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| Molecular Weight | 302.12 | CAS No. | 17440-83-4 | |
| Solubility (25°C)* | In vitro | DMSO | 100 mg/mL (330.99 mM) | |
| Water | 5 mg/mL (16.54 mM) | |||
| Ethanol | 0 mg/mL (0.0 mM) | |||
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* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.) |
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Preparing Stock Solutions
Biological Activity
| Description | Amiloride HCl dihydrate(MK-870 hydrochloride dihydrate) is a potent epithelial sodium channel (ENaC) blocker, used in the management of hypertension and congestive heart failure. | |
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| In vitro | Amiloride also induces the dephosphorylation of P13K (phosphatidylinositol 3-kinase) and PDK-1 (phosphoinositide-dependent kinase-1) kinases along with PTEN (phosphatase and tensin homolog deleted on chromosome 10) and PP1 alpha phosphatases. Amiloride inhibits phosphorylation of kinases and phosphatases by competing with ATP. Amiloride, which causes little or no cytotoxicity by itself, enhances TRAIL-induced apoptosis. [1] Amiloride precludes the alkalinization and in parallel inhibit cellular proliferation. Amiloride directly inhibits autophosphorylation of the EGF receptor. [2] Amiloride significantly enhances recovery to a maximum of 39%, 88%, and 78% for force, +dF/dt, and -dF/dt, respectively. [3] Amiloride, a frequently used inhibitor of Na+/H+ exchange, rapidly inhibits phorbol ester-stimulated protein phosphorylation in vivo and protein kinase C-mediated phosphorylation in vitro, both with potency similar to that with which Amiloride inhibits Na+/H+ exchange. Amiloride blocks phorbol ester-induced adhesion of HL-60 cells (adhesion being a property indicative of the differentiated state), but dimethylamiloride (as well as ethylisopropylamiloride, another very potent amiloride analog) does not. [4] Amiloride inhibits the ouabain-sensitive rate of oxygen consumption (QO2) of a suspension of rabbit intact proximal tubules in the presence of different concentrations of extracellular sodium. [5] |
Protocol (from reference)
References
Selleck's Amiloride HCl dihydrate has been cited by 11 publications
| Propafenone facilitates mitochondrial-associated ferroptosis and synergizes with immunotherapy in melanoma [ J Immunother Cancer, 2024, 12(11)e009805] | PubMed: 39581704 |
| Versatile Nano-PROTAC-Induced Epigenetic Reader Degradation for Efficient Lung Cancer Therapy [ Adv Sci (Weinh), 2022, 9(29):e2202039] | PubMed: 35988145 |
| The sodium/glucose cotransporters as potential therapeutic targets for CF lung diseases revealed by human lung organoid swelling assay [ Mol Ther Methods Clin Dev, 2022, 24:11-19] | PubMed: 34977268 |
| Endocytic pathway inhibition attenuates extracellular vesicle-induced reduction of chemosensitivity to bortezomib in multiple myeloma cells [ Theranostics, 2021, 11(5):2364-2380] | PubMed: 33500730 |
| Endocytic pathway inhibition attenuates extracellular vesicle-induced reduction of chemosensitivity to bortezomib in multiple myeloma cells [ Theranostics, 2021, 11(5):2364-2380] | PubMed: 33500730 |
| Modification of Metal-Organic Framework Nanoparticles Using Dental Pulp Mesenchymal Stem Cell Membranes to Target Oral Squamous Cell Carcinoma [ J Colloid Interface Sci, 2021, 601:650-660] | PubMed: 34091312 |
| Pore-forming alpha-hemolysin efficiently improves the immunogenicity and protective efficacy of protein antigens [ PLoS Pathog, 2021, 17(7):e1009752] | PubMed: 34288976 |
| Evaluation of both exonic and intronic variants for effects on RNA splicing allows for accurate assessment of the effectiveness of precision therapies [ PLoS Genet, 2020, 16(10):e1009100] | PubMed: 33085659 |
| Cellular uptake pathways of sepiolite nanofibers and DNA transfection improvement. [Castro-Smirnov FA, et al. Sci Rep, 2017, 7(1):5586] | PubMed: 28717157 |
| Signaling pathways of a structural analogue of apelin-12 involved in myocardial protection against ischemia/reperfusion injury [ Peptides, 2015, 73:67-76] | PubMed: 26348269 |
RETURN POLICY
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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.
NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.