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Formula | C21H14N2Na2O9S2 |
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Molecular Weight | 548.45 | CAS No. | 20324-87-2 | |
Solubility (25°C)* | In vitro | DMSO | 100 mg/mL (182.33 mM) | |
Water | 22 mg/mL (40.11 mM) | |||
Ethanol | Insoluble | |||
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.) |
Description | AMI-1 is a potent and specific Histone Methyltransferase (HMT) inhibitor with IC50 of 3.0 μM and 8.8 μM for yeast Hmt1p and human PRMT1, respectively. | ||||
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Targets |
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In vitro | In HeLa cells, AMI-1 inhibits methylation levels of GFP-Npl3 fusion and endogenous PRMT1-like activity. AMI-1 also inhibits nuclear receptor-mediated transactivation of a luciferase reporter in MCF7 cells. [1] In addition, AMI-1 inhibits HIV-1 RT polymerase activity with IC50 of 5 μM and inhibits DNA binding to HIV-1 RT with Kd of 2 μM. [2] In INS-1 cells, AMI-1 improves INS-1 cell function and mediates translocations of FOXO1 and PDX-1 intracellularly by regulating FOXO1 phosphorylation and methylation. [4] | ||||
In vivo | In chronic AIPI rats, AMI-1 (5 μg/rat) ameliorates COX2 expression and asthmatic indexes, and decreases the airway and alveoli lesions, mucus secretion, and collagen deposition in lungs. [3] |
Animal Study:[3] |
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Data from [Data independently produced by , , Nitric Oxide, 2017, 1-10]
PRMT1 reverts the immune escape of necroptotic colon cancer through RIP3 methylation [ Cell Death Dis, 2023, 14(4):233] | PubMed: 37005412 |
Methylation of HBP1 by PRMT1 promotes tumor progression by regulating actin cytoskeleton remodeling [ Oncogenesis, 2022, 11(1):45] | PubMed: 35941115 |
PRMT1 enhances oncogenic arginine methylation of NONO in colorectal cancer [ Oncogene, 2021, 40(7):1375-1389] | PubMed: 33420374 |
PRMT1 enhances oncogenic arginine methylation of NONO in colorectal cancer [ Oncogene, 2021, 10.1038/s41388-020-01617-0] | PubMed: 33420374 |
Hypoxia Stimulates SUMOylation-Dependent Stabilization of KDM5B [ Front Cell Dev Biol, 2021, 9:741736] | PubMed: 34977006 |
Hypoxia stimulates SUMOylation-dependent stabilization of KDM5B [ Research Square, 2021, 10.21203/rs.3.rs-620216/v1] | PubMed: None |
CARM1 Methylates GAPDH to Regulate Glucose Metabolism and Is Suppressed in Liver Cancer. [ Cell Rep, 2018, 24(12):3207-3223] | PubMed: 30232003 |
The asymmetric dimethylarginine-mediated inhibition of nitric oxide in the rostral ventrolateral medulla contributes to regulation of blood pressure in hypertensive rats. [Tan X, et al. Nitric Oxide, 2017, 67:58-67] | PubMed: 28392446 |
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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.
NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.