Alpinetin

Catalog No.S3875 Batch:S387503

Technical Data

Formula

C₁₆H₁₄O₄

Molecular Weight

270.28

CAS No.

36052-37-6

Solubility (25°C)*

In vitro

DMSO

54 mg/mL (199.79 mM)

Water

Insoluble

Ethanol

Insoluble

In vivo (Add solvents to the product individually and in order)

Clear solution	5% DMSO 1 95% Corn oil	0.3mg/ml	Taking the 1 mL working solution as an example, add 50 μL of 6 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O	0.65mg/ml	Taking the 1 mL working solution as an example, add 50 μL of 13 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	Alpinetin, a composition of Alpinia katsumadai Hayata, has been reported to have a number of biological properties, such as antibacterial, antitumor and other important therapeutic activities. Alpinetin is a flavonoid isolated from Alpinia katsumadai Hayata, activates activates PPAR-γ, with potent anti-inflammatory activity.
In vitro	Alpinetin markedly inhibits the LPS- induced TNF-α, IL-6 and IL-1β production both in vitro and vivo. Furthermore, alpinetin blocks the phosphorylation of IκBα protein, p65, p38 and extracellular signal-regulated kinase (ERK) in LPS stimulated RAW 264.7 cells^[1]. Alpinetin can suppress the proliferation and invasiveness of GSCs and induce apoptosis in GSCs^[2].
In vivo	Alpinetin attenuates lung histopathologic changes in mouse models^[1].

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines Raw 264.7 cells Concentrations 0–300 μg/ml Incubation Time 1 h Method Cell viability is evaluated by MTT assay. Briefly, 100 μl cells are plated at a density of 4×10⁵ cells/ml in 96-well plates in a 37 ℃, 5% CO2 incubator for 1 h. Then the cells are treated with 50 μl of alpinetin of different concentrations (0-300 μg/ml) for 1 h, followed by stimulation with 50 μl LPS (4 μg/ml). The total volume of each well is 200 μl, and the concentrations of Alpinetin and LPS are four times diluted finally. After 18 h of LPS stimulation, 20 μl MTT (5 mg/ml) is added to each well, and the cells are further incubated for an additional 4 h. The supernatant is removed and the formation of formazan is resolved with 150 μl/well of DMSO. The optical density is measured at 570 nm on a microplate reader.
Animal Study:^{^[1]}	Animal Models Male BALB/c mice Dosages 50 mg/kg Administration i.p.

Cell Assay:^{^[1]}

Cell lines
Raw 264.7 cells
Concentrations
0–300 μg/ml
Incubation Time
1 h
Method
Cell viability is evaluated by MTT assay. Briefly, 100 μl cells are plated at a density of 4×10⁵ cells/ml in 96-well plates in a 37 ℃, 5% CO2 incubator for 1 h. Then the cells are treated with 50 μl of alpinetin of different concentrations (0-300 μg/ml) for 1 h, followed by stimulation with 50 μl LPS (4 μg/ml). The total volume of each well is 200 μl, and the concentrations of Alpinetin and LPS are four times diluted finally. After 18 h of LPS stimulation, 20 μl MTT (5 mg/ml) is added to each well, and the cells are further incubated for an additional 4 h. The supernatant is removed and the formation of formazan is resolved with 150 μl/well of DMSO. The optical density is measured at 570 nm on a microplate reader.

Animal Study:^{^[1]}

Animal Models
Male BALB/c mice
Dosages
50 mg/kg
Administration
i.p.

References

Selleck's Alpinetin has been cited by 1 publication

Inhibitory Effect of Tangeretin and Cardamonin on Human Intestinal SGLT1 Activity In Vitro and Blood Glucose Levels in Mice In Vivo [ Nutrients, 2021, 13(10)3382]	PubMed: 34684383

Inhibitory Effect of Tangeretin and Cardamonin on Human Intestinal SGLT1 Activity In Vitro and Blood Glucose Levels in Mice In Vivo [ Nutrients, 2021, 13(10)3382]

PubMed: 34684383

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.