α-Hederin

Catalog No.S3914 Batch:S391401

Technical Data

Formula	C₄₁H₆₆O₁₂
Molecular Weight	750.96	CAS No.	27013-91-8
Solubility (25°C)*	In vitro	DMSO	100 mg/mL (133.16 mM)
		Water	Insoluble

* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	α-hederin is a water-soluble pentacyclic triterpenoid saponin which has shown hemolytic and apoptotic properties.
In vitro	α-hederin shows strong inhibitory activity on the growth of breast cancer cells and induces apoptosis in these cells. α-hederin induces depolarization of mitochondrial membrane potential which releases Apaf-1 and cytochrome c from the intermembrane space into the cytosol, where they promote caspase-3 and caspase-9 activation^[1].
In vivo	After a single i.v. (2 mg/kg) administration, the mean plasma clearance (CL), volume of distribution (V_SS) and elimination half-life (t_1/2) of α-hederin in rtas are 0.24 L/h/kg, 0.25 L/kg and 2.67 h, respectively. The oral bioavailability (F) of α-hederin in rats is about 0.14%, which might result from the poor intestinal absorption and/or extensive biliary excretion. α-Hederin decreases the hepatotoxicity of cadmium in mice by inducing hepatic metallothionein I/II, and the mechanism partly involved the upregulation of metal-lothionein expression mediated by TNF-α and IL-6^[2].

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines The human breast cancer cell lines MCF-7 and MDA-MB-231 Concentrations 0.08, 0.4, 2 and 10 μg/ml Incubation Time 12, 24 and 48 h Method The MTT assay is used to measure the inhibition of growth by α-hederin in breast cancer cell lines. Briefly, 5×10³ cells are seeded into a 96-well plate in triplicate and 8 h later α-hederin is added into the wells at the indicated final concentrations (0.08, 0.4, 2 and 10 μg/ml), while cells cultured in medium with 0.05% DMSO as a negative control. After incubation with α-hederin for 12, 24 and 48 h, the medium in each well is replaced with 20 μl of MTT at 5 mg/ml final concentration, and 4 h later 150 μl DMSO/well is added to dissolve the formed violet formazan crystals within metabolically viable cells. The plates are incubated at room temperature for 15 min and then read at 490 nm with a microplate reader. The percentage of growth inhibition is calculated as (OD of the control-OD of the experiment samples)/OD of the control × 100.
Animal Study:^{^[2]}	Animal Models Sprague-Dawley (SD) rats Dosages 10 mg/kg (p.o.) or 2 mg/kg (i.v.) Administration p.o. or i.v.

Cell Assay:^{^[1]}

Cell lines
The human breast cancer cell lines MCF-7 and MDA-MB-231
Concentrations
0.08, 0.4, 2 and 10 μg/ml
Incubation Time
12, 24 and 48 h
Method
The MTT assay is used to measure the inhibition of growth by α-hederin in breast cancer cell lines. Briefly, 5×10³ cells are seeded into a 96-well plate in triplicate and 8 h later α-hederin is added into the wells at the indicated final concentrations (0.08, 0.4, 2 and 10 μg/ml), while cells cultured in medium with 0.05% DMSO as a negative control. After incubation with α-hederin for 12, 24 and 48 h, the medium in each well is replaced with 20 μl of MTT at 5 mg/ml final concentration, and 4 h later 150 μl DMSO/well is added to dissolve the formed violet formazan crystals within metabolically viable cells. The plates are incubated at room temperature for 15 min and then read at 490 nm with a microplate reader. The percentage of growth inhibition is calculated as (OD of the control-OD of the experiment samples)/OD of the control × 100.

Animal Study:^{^[2]}

Animal Models
Sprague-Dawley (SD) rats
Dosages
10 mg/kg (p.o.) or 2 mg/kg (i.v.)
Administration
p.o. or i.v.

References

RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.

SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.