alpha-Asarone

Catalog No.S4772 Batch:S477201

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Technical Data

Formula

C12H16O3

Molecular Weight 208.25 CAS No. 2883-98-9
Solubility (25°C)* In vitro DMSO 41 mg/mL (196.87 mM)
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description Alpha (α)-asarone (trans-Asarone) is one of the main pharmacologically active compounds present in Acorus calamus Linn (Acoraceae), Acorus tatarinowii Schott (Acoraceae), and Acorus gramineus Solander. It has anticonvulsant, neuroprotective, anxiolytic and nootropic effects.
In vitro α-asarone possesses a endothelium-dependent vasodilation property, and in normal HUVEC-derived EA.hy926 cells, α-asarone has been shown to upregulate the expression of phosphorylated eNOS (Ser1177), causing an increase in the production of NO. It protects against injury to endothelial cells by attenuating Ang II-mediated changes in intracellular ROS/NO[1].
In vivo Alpha-Asarone treatment alleviates locomotor hyperactivity in Fmr1 KO mice. It improves m1 mAChR expression and up-regulated the ACh levels in the striatum. Its treatment also attenuates the increased striatal AChE activity in Fmr1 KO mice. Alpha-asarone has been reported to have a beneficial effectin cognitive impairment induced by dysfunction of the cholinergicsystem in the brain through inhibition of AChE activity[2]. Of all the doses tested (2, 10, 40, 80 and 120 mg/kg) in rats, 10 mg/kg α-Asarone improves the quality of sleep, as indicated by an increased NREM bout duration, reduced arousal index, and decreased bout frequencies of NREM sleep and wakefulness. A marginal decrease in the hypothalamic and body temperatures is also observed. Higher doses, on the other hand, not only reduce the quantity and quality of sleep, but also produce hypothermia[3].

Protocol (from reference)

Cell Assay:[1]
  • Cell lines

    EA.hy926 cell line

  • Concentrations

    10, 50, 100 µmol/L

  • Incubation Time

    1 h

  • Method

    EA.hy926 cells are subcultured at 50,000/well in 96-well plates as in the experiment above and pretreated with α-asarone at different concentrations (10, 50, and 100 μmol/L) for 1 h. Subsequently, Ang II (0.1 μmol/L) is added to the cultures and incubated for 24 h at 37°C. Thereafter, the cells are loaded with the fluorescent probes for 30 min, and then the extracellular residual dyes are washed away before detection.

Animal Study:[2]
  • Animal Models

    Fmr1 knockout (KO) mice (male, 6–8 weeks of age)

  • Dosages

    10 mg/kg

  • Administration

    i.p.

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.