BVT-948

Catalog No.S9829 Batch:S982901

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Technical Data

Formula

C14H11NO3
Molecular Weight 241.24 CAS No. 39674-97-0
Solubility (25°C)* In vitro DMSO 48 mg/mL (198.97 mM)
Ethanol 5 mg/mL (20.72 mM)
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description BVT 948 is a irreversible, noncompetitive and cell-permeable protein tyrosine phosphatase（PTP） inhibitor with IC50s of 0.9 μM, 1.7 μM, 0.09 μM, 1.5 μM and 0.7 μM for PTP1B, TCPTP, SHP-2, LAR and YopH respectively.
Targets
SHP-2 [1]
(Cell-free assay)		 YopH [1]
(Cell-free assay)		 PTP1B [1]
(Cell-free assay)		 LAR [1]
(Cell-free assay)		 TCPTP [1]
(Cell-free assay)
0.09 μM 0.7 μM 0.9 μM 1.5 μM 1.7 μM
In vitro

In L6 myotubes, PTP1B is oxidized further to the irreversible sulfinic and sulfonic forms with BVT.948. BVT.948 is able to increase tyrosine phosphorylation of cellular proteins, including the insulin receptor. BVT.948 inhibits several other PTPs tested and also was efficient at inhibiting several cytochrome P450 (P450) isoforms.[1]
In vivo

BVT.948 enhances insulin tolerance tests in ob/ob mice, therefore apparently enhancing insulin sensitivity. BVT.948 can significantly enhance glucose clearance from the blood stream in response to insulin. [1]

Protocol (from reference)

Cell Assay:

[1]
  • Cell lines

    L6 myotubes

  • Concentrations

    25 μM

  • Incubation Time

    30 min

  • Method

    L6 myotubes are starved of serum overnight and stimulated with insulin or treated with 25 M BVT.948 after a 30-min preincubation with (dark gray bars) or without (light gray bars) 100 nM wortmannin. After washing, glucose uptake was measured.
Animal Study:

[1]
  • Animal Models

    ob/ob mice

  • Dosages

    0.3 μmol/kg, 3 μmol/kg

  • Administration

    i.p.

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.