AGK2

Catalog No.S7577 Batch:S757703

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Technical Data

Formula

C23H13Cl2N3O2
Molecular Weight 434.27 CAS No. 304896-28-4
Solubility (25°C)* In vitro DMSO (warmed with 50ºC water bath) 4 mg/mL (9.21 mM)
Water Insoluble
Ethanol Insoluble
In vivo (Add solvents to the product individually and in order)
Clear solution
8%DMSO 30%PEG300 62%ddH2O
1.4mg/ml Taking the 1 mL working solution as an example, add 80 μL of 17.5 mg/ml clarified DMSO stock solution to 300 μL of PEG 300, mix evenly to clarify it; then continue to add 620 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description AGK2 is a potent, and selective SIRT2 inhibitor with IC50 of 3.5 μM that minimally affects either SIRT1 or SIRT3 at 10-fold higher levels.
Targets
SIRT2 [1]
(Cell-free assay)
3.5 μM
In vitro In SIRT2-myc-expressing HeLa cells, AGK2 effectively inhibits the activity of SIRT2, and increases acetylated tubulin. AGK2 protects dopaminergic neurons from α-Syn–induced toxicity in primary midbrain cultures. [1] AGK2 induces both necrosis and caspase-3-dependent apoptosis in C6 glioma cells. [2] SIRT2 also decreases merlin-mutant viability of mouse schwann cells (MSCs) without substantially reducing wild-type MSC viability. [3]
In vivo In drosophila model of parkinson's disease, AGK2 rescues α-Syn–mediated toxicity and modify aggregation. [1]

Protocol (from reference)

Animal Study:

[1]
  • Animal Models

    Drosophila model of parkinson

  • Dosages

    1mM

  • Administration

    --

Customer Product Validation

Data from [Data independently produced by , , Cell Physiol Biochem, 2018, 46(2):451-460]

Data from [Data independently produced by , , Mol Cancer Res, 2018, doi:10.1158/1541-7786.MCR-18-0637]

Data from [Data independently produced by , , J Biol Chem, 2014, 289(9): 6054-66 ]

Data from [Data independently produced by , , Cancer Biol Ther, 2018, 19(9):835-846]

Selleck's AGK2 has been cited by 33 publications

NAD+ precursors promote the restoration of spermatogenesis in busulfan-treated mice through inhibiting Sirt2-regulated ferroptosis [ Theranostics, 2024, 14(6):2622-2636] PubMed: 38646657
Mitochondrial aldehyde dehydrogenase-2 coordinates the hydrogen sulfide - AMPK axis to attenuate high glucose-induced pancreatic β-cell dysfunction by glutathione antioxidant system [ Redox Biol, 2024, 69:102994] PubMed: 38128451
The SIRT2-AMPK axis regulates autophagy induced by acute liver failure [ Sci Rep, 2024, 14(1):16278] PubMed: 39009648
SIRT2 inhibition protects against cardiac hypertrophy and ischemic injury [ Elife, 2023, 12e85571] PubMed: 37728319
SIRT2 inhibition protects against cardiac hypertrophy and ischemic injury [ Elife, 2023, 12e85571] PubMed: 37728319
SIRT2 Inhibition Rescues Neurodegenerative Pathology but Increases Systemic Inflammation in a Transgenic Mouse Model of Alzheimer's Disease [ J Neuroimmune Pharmacol, 2023, 18(3):529-550] PubMed: 37698780
Continuous Fluorescent Sirtuin Activity Assay Based on Fatty Acylated Lysines [ Int J Mol Sci, 2023, 24(8)7416] PubMed: 37108579
VRK1 Kinase Activity Modulating Histone H4K16 Acetylation Inhibited by SIRT2 and VRK-IN-1 [ Int J Mol Sci, 2023, 24(5)4912] PubMed: 36902348
High-Content and High-Throughput Clonogenic Survival Assay Using Fluorescence Barcoding [ Cancers -Basel), 2023, 15(19)4772] PubMed: 37835466
AGK2 pre-treatment protects against thioacetamide-induced acute liver failure via regulating the MFN2-PERK axis and ferroptosis signaling pathway [ Hepatobiliary Pancreat Dis Int, 2023, S1499-3872(23)00037-1] PubMed: 36966125

RETURN POLICY
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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.