Actinomycin D (Dactinomycin)

Catalog No.S8964 Batch:S896401

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Technical Data

Formula

C62H86N12O16
Molecular Weight 1255.42 CAS No. 50-76-0
Solubility (25°C)* In vitro DMSO 100 mg/mL (79.65 mM)
Water Insoluble
Ethanol Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description Actinomycin D (Dactinomycin, Act D, RASP-101) is a significant polypeptide antibiotic isolated from soil bacteria of the genus Streptomyces. Actinomycin D (Dactinomycin) inhibits DNA repair and rests the cell cycle at G1 phase with IC50 of 0.42 μM and 0.4 nM, respectively. Actinomycin D is an autophagy activator, induces p53-independent cell death and prolongs survival in high-risk chronic lymphocytic leukemia.This product is a hazardous chemical (acute toxicity/flammable/skin corrosive). Please use it while wearing a protective face mask, gloves, and clothing.
Targets
autophagy [3] cell cycle [1]
(Cell-based assay)		 DNA repair [2]
(Cell-free assay)
0.4 nM 0.42 μM
In vitro

Actinomycin D markedly reduces the SMC proliferation via the inhibition of BrdU incorporation at 80 nM. This is further supported by the G1-phase arrest using a flowcytometric analysis. The protein expression levels of proliferating cell nuclear antigen (PCNA), focal adhesion kinase (FAK), and Raf are all suppressed by actinomycin D. Extracellular signalregulated kinases (Erk) involved in cell-cycle arrest are found to be increased by actinomycin D.[1]
In vivo

Actinomycin D targets survival proteins TOSO, BCL2 and MCL1 and is active in two different mouse models that are characterized by either unmutated B-cell receptor or inactive p53 function, both of which are known negative prognostic factors in CLL.[3]

Protocol (from reference)

Cell Assay:

[1]
  • Cell lines

    A10 cells (Vascular SMC)

  • Concentrations

    80 nM, 800 nM, 8 μM

  • Incubation Time

    18-24 h

  • Method

    Cultured SMC are starved for 24 h followed by incubation with various doses of actinomycin D at 37℃. Drug treatment is carried out for 18-24 h. Since actinomycin D is dissolved in 0.1% DMSO, a vehicle control containing DMSO is also included.
Animal Study:

[3]
  • Animal Models

    C57BL/6 wild-type mice engrafted with tumor cells from Em-TCL-1 transgenic mice

  • Dosages

    0.06 mg/kg

  • Administration

    IP

Selleck's Actinomycin D (Dactinomycin) has been cited by 106 publications

YTHDF2 in peritumoral hepatocytes mediates chemotherapy-induced antitumor immune responses through CX3CL1-mediated CD8+ T cell recruitment [ Mol Cancer, 2024, 23(1):186] PubMed: 39237909
Acetylation-dependent regulation of core spliceosome modulates hepatocellular carcinoma cassette exons and sensitivity to PARP inhibitors [ Nat Commun, 2024, 15(1):5209] PubMed: 38890388
Cbx4 SUMOylates BRD4 to regulate the expression of inflammatory cytokines in post-traumatic osteoarthritis [ Exp Mol Med, 2024, 10.1038/s12276-024-01315-x] PubMed: 39349832
Angiotension II directly bind P2X7 receptor to induce myocardial ferroptosis and remodeling by activating human antigen R [ Redox Biol, 2024, 72:103154] PubMed: 38626575
m6A-dependent upregulation of DDX21 by super-enhancer-driven IGF2BP2 and IGF2BP3 facilitates progression of acute myeloid leukaemia [ Clin Transl Med, 2024, 14(4):e1628] PubMed: 38572589
Energy stress-induced circDDX21 promotes glycolysis and facilitates hepatocellular carcinogenesis [ Cell Death Dis, 2024, 15(5):354] PubMed: 38773094
The m6A reader HNRNPC promotes glioma progression by enhancing the stability of IRAK1 mRNA through the MAPK pathway [ Cell Death Dis, 2024, 15(6):390] PubMed: 38830885
Alterations of ceramide synthesis induce PD-L1 internalization and signaling to regulate tumor metastasis and immunotherapy response [ Cell Rep, 2024, 43(8):114532] PubMed: 39046874
Targeting high circDNA2v levels in colorectal cancer induces cellular senescence and elicits an anti-tumor secretome [ Cell Rep, 2024, 43(4):114111] PubMed: 38615319
N6-methyladenosine-modified SRPK1 promotes aerobic glycolysis of lung adenocarcinoma via PKM splicing [ Cell Mol Biol Lett, 2024, 29(1):106] PubMed: 39095708

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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