|
Toll Free: (877) 796-6397 -- USA and Canada only -- |
Fax: +1-832-582-8590 Orders: +1-832-582-8158 |
Tech Support: +1-832-582-8158 Ext:3 Please provide your Order Number in the email. |
Technical Data
| Formula | C4H5NO4S.K |
|||
| Molecular Weight | 202.25 | CAS No. | 55589-62-3 | |
| Solubility (25°C)* | In vitro | DMSO | 40 mg/mL (197.77 mM) | |
| Water | 40 mg/mL (197.77 mM) | |||
| Ethanol | Insoluble | |||
|
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.) |
||||
Preparing Stock Solutions
Biological Activity
| Description | Acesulfame potassium is a non-nutritive sweetener. |
|---|---|
| In vitro | Acesulfame potassium activates two members of the human TAS2R family (hTAS2R43 and hTAS2R44) to stimulate bitter taste. Acesulfame potassium elicited robust elevation of cytosolic Ca2+ in hTAS2R44-expressing cells, with a threshold value of activation of 0.25 mM and an EC50 value of 2.5 mM. Acesulfame potassium elicited response of hTAS2R43-expressing cells with a threshold value of 3.1 mM and an estimated EC50 value ﹥10 mM [1] Acesulfame potassium acts directly on the pancreatic islets and potentiates glucose-induced insulin release. Acesulfame potassium (1.0-15.0 mM) augmented insulin release from islets incubated in the presence of 7.0 mM d-glucose. [2] Acesulfame Potassium enhanced glucose absorption via activating sweet taste receptors in the enterocyte to translocate GLUT2 to the apical membrane through the PLC βII. In Caco-2 and RIE-1 cells, Acesulfame potassium (10 mM) increased glucose uptake by 20-30 % when incubated for 10 min with glucose >25 mM. [3] Acesulfame potassium increased the contractile response of isolated rat detrusor muscle strips via increased extracellular Ca2+ influx. Acesulfame potassium (10-7 M to 10-2 M) enhanced the contractile response to 10 Hz EFS compared to control. The atropine-resistant response to EFS is marginally increased by Acesulfame potassium (10-6 M). Acesulfame potassium (10-6 M) increased the maximum contractile response to α, β methylene ATP by 35% and to KCl by 12%. Acesulfame potassium (10-6 M) increased the log EC50 from -2.7 to -3.03. [4] |
| In vivo | Acesulfame potassium acts on two members of the TAS1R family of G-protein-coupled receptors (TAS1R2 and TAS1R3) to stimulate sweet taste. Selective elimination of T1R-subunits differentially abolishes de tection and perception of these two taste modalities. [5] Acesulfame potassium can also induce insulin secretion in rats. Injection of Acesulfame potassium (150 mg/kg body weight) increased the plasma insulin concentration at 5 min from 27.3 mU/mL to 58.6 mU/mL. Infusion of Acesulfame potassium (20 mg/kg body weight/min) for one hour maintained the insulin concentration at a high level (about 85-100 mU/mL). When using different amounts of Acesulfame potassium, the insulin secretion is stimulated in a dose-dependent fashion. [6] Oral administration of Acesulfame potassium (60, 450, 1500 and 2250 mg/kg body weight) induced a significant increase in the frequency of cellular damage and chromosome aberrations in the Bone marrow cells isolated from mice femora. [7] Oral administration of Acesulfame potassium at the concentration of 150, 300, and 600 mg/kg body weight is found to induce DNA damage in bone marrow cells of mice with a minimum effective concentration (MEC) value of 150 mg/kg in the comet assay. [8] |
Protocol (from reference)
References
RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.
SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.
NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.