Ac-FLTD-CMK

Catalog No.S9817 Batch:S981702

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Technical Data

Formula

C26H37ClN4O8
Molecular Weight 569.05 CAS No. 2376255-48-8
Solubility (25°C)* In vitro DMSO 100 mg/mL (175.73 mM)
Ethanol 100 mg/mL (175.73 mM)
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description Ac-FLTD-CMK is a specific inhibitor for inflammatory caspases. Ac-FLTD-CMK inhibits gasdermin D (GSDMD) cleavage by caspases-1, -4, -5, and -11 in vitro. Ac-FLTD-CMK is effective against caspases-1, caspases-4 and caspases-5 with IC50 of 46.7 nM, 1.49 μM and 329 nM, respectively.
Targets
caspases-1 [1]
(Cell-free assay)		 caspases-5 [1]
(Cell-free assay)		 caspases-4 [1]
(Cell-free assay)
46.7 nM 329 nM 1.49 μM
In vitro

A GSDMD-derived inhibitor, N-acetyl-Phe-Leu-Thr-Asp-chloromethylketone (Ac-FLTD-CMK), inhibits GSDMD cleavage in vitro and suppresses pyroptosis downstream of both canonical and noncanonical inflammasomes.[1]
In vivo

Ac-FLTD-CMK inhibits pyroptosis and exerts neuroprotective effect in a mice model of traumatic brain injury.[2]

Protocol (from reference)

Cell Assay:

[1]
  • Cell lines

    BMDM cells

  • Concentrations

    10 μM

  • Incubation Time

    30 mins

  • Method

    To observe ASC speck formation, BMDM cells expressing cerulean-ASC are plated at 2 x 104 /well in 96-well plates the day prior to experiment. Cells are primed with 200 ng/ml LPS for 4 hours before stimulating with either 5 mM ATP or 10 μM nigericin to activate the NLRP3 inflammasome, in the presence or absence of 10 μM z-VAD-FMK, Ac-FLTD-CMK, or MCC950. After 30 mins, the cerulean-ASC speck formation is observed with a Leica DMIL fluorescence microscopy.
Animal Study:

[2]
  • Animal Models

    10–12 week-old male C57BL/6 mice

  • Dosages

    0.5 μg for each mouse

  • Administration

    ICV

Selleck's Ac-FLTD-CMK has been cited by 2 publications

Characterization of regulated cancer cell death pathways induced by the different modalities of non-thermal plasma treatment [ Cell Death Discov, 2024, 10(1):416] PubMed: 39349444
Double-Edged Sword Effect of Pyroptosis: The Role of Caspase-1/-4/-5/-11 in Different Levels of Apical Periodontitis [ Biomolecules, 2022, 12(11)1660] PubMed: 36359010

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.