ABTL-0812

Catalog No.S9611 Batch:S961101

Technical Data

Formula

C₁₈H₃₂O₃

Molecular Weight

296.44

CAS No.

57818-44-7

Solubility (25°C)*

In vitro

In vivo (Add solvents to the product individually and in order)

Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O	5.0mg/ml	Taking the 1 mL working solution as an example, add 50 μL of 100 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil	2.5mg/ml	Taking the 1 mL working solution as an example, add 50 μL of 50 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Biological Activity

Description

ABTL0812 (α-Hydroxylinoleic acid, LP-10218, SCLN-0812) inhibits Akt/mTOR axis by inducing the overexpression of TRIB3 and activating autophagy in lung squamous carcinoma cell lines. ABTL0812 also induces AMPK activation and ROS accumulation.

Targets

Akt ^[1]

mTOR ^[1]

In vitro

ABTL0812 inhibits Akt/mTOR axis by inducing the overexpression of TRIB3 and activating autophagy in lung squamous carcinoma cell lines. Furthermore, treatment with ABTL0812 also induces AMPK activation and ROS accumulation. Moreover, combination of ABTL0812 with chemotherapy markedly increases the therapeutic effect of chemotherapy without increasing toxicity. Combination of ABTL0812 and chemotherapy induces nonapoptotic cell death mediated by TRIB3 activation and autophagy induction.^[1]

In vivo

ABTL0812 enhances antitumor activity both in adenocarcinomas and squamous lung xenografts.^[1]

Density

1.0 g/mL

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines Human adenocarcinoma cell lines (A549, NCI-H1975), squamous-cell carcinoma (NCI-H520, NCI-H157) Concentrations 0–200 μM Incubation Time 72 h Method Briefly, for studies, subconfluent cultures of adenocarcinoma lung cancer cells are incubated for 72 hr in the presence of an increasing concentration of ABTL0812 (0–200 μM), or the combination of increasing doses with ABTL0812 20 μM. For other studies, subconfluent cultures of adenocarcinoma and squamous lung cancer cell lines are incubated for 48 hr in the presence of an increasing concentration or the combination of increasing doses with sub-IC50 concentrations of ABTL0812 (5–30 μM).
Animal Study:^{^[1]}	Animal Models 5-week-old female athymic nude Foxn 1nu nu/nu mice Dosages 30 mg/kg, 120 mg/kg Administration Oral gavage

Cell Assay:^{^[1]}

Cell lines
Human adenocarcinoma cell lines (A549, NCI-H1975), squamous-cell carcinoma (NCI-H520, NCI-H157)
Concentrations
0–200 μM
Incubation Time
72 h
Method
Briefly, for studies, subconfluent cultures of adenocarcinoma lung cancer cells are incubated for 72 hr in the presence of an increasing concentration of ABTL0812 (0–200 μM), or the combination of increasing doses with ABTL0812 20 μM. For other studies, subconfluent cultures of adenocarcinoma and squamous lung cancer cell lines are incubated for 48 hr in the presence of an increasing concentration or the combination of increasing doses with sub-IC50 concentrations of ABTL0812 (5–30 μM).

Animal Study:^{^[1]}

Animal Models
5-week-old female athymic nude Foxn 1nu nu/nu mice
Dosages
30 mg/kg, 120 mg/kg
Administration
Oral gavage

References

[1] Anna López-Plana, et al. Int J Cancer. 2020 Aug 15;147(4):1163-1179.

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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