Thiazovivin

Catalog No.S1459 Batch:S145905

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Technical Data

Formula

C15H13N5OS
Molecular Weight 311.36 CAS No. 1226056-71-8
Solubility (25°C)* In vitro DMSO 63 mg/mL (202.33 mM)
Water Insoluble
Ethanol Insoluble
In vivo (Add solvents to the product individually and in order)
Clear solution
30%PEG400 0.5%Tween80 5%propylene glycol
30.0mg/ml Taking the 1 mL working solution as an example, add 300 μL of 100 mg/ml clarified PEG400 stock solution to 5 μL of Tween80, mix evenly to clarify it; add 50 μL Propylene glycol to the above system, mix evenly to clarify it; then continue to add 645 μL ddH2O to adjust the volume. to 1 mL. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description Thiazovivin is a novel ROCK inhibitor with IC50 of 0.5 μM in a cell-free assay, promotes hESC survival after single-cell dissociation.
Targets
ROCK [1]
(Cell-free assay)
~0.5 μM
In vitro

Although displaying little impact on cell proliferation, Thiazovivin treatment significantly enhances the survival of human embryonic stem cells (hESCs) after enzymatic dissociation more than 30-fold, while homogenously maintaining pluripotency with the characteristic colony morphology, expression of typical pluripotency markers such as alkaline phosphatase (ALP), and normal karyotype. Dissociated hESCs treated with Thiazovivin display dramatically increased adhesion to matrigel- or laminin-coated plates but not to gelatin-coated plates within a few hours. Thiazovivin treatment increases cell-ECM adhesion-mediated β1 integrin activity, which synergizes with growth factors to promote cell survival. In addition to activating integrin, Thiazovivin but not Tyrintegin (Ptn) protects hESCs from death in the absence of ECM in suspension through E-cadherin-mediated cell-cell interaction. Thiazovivin treatment potently inhibits endocytosis of E-cadherin, consequently stabilizing E-cadherin on the cell surface and leading to reestablishment of cell-cell interaction, which is essential for hESC survival in ECM-free conditions. Thiazovivin but not Tyrintegin (Ptn) at 2 μM inhibits Rho-associated kinase (ROCK) activity and protects hESCs at a similar level as the widely used selective ROCK inhibitor Y-27632 at 10 μM, suggesting that Rho-ROCK signaling regulates cell-ECM and cell-cell adhesion. [1]

Thiazovivin at 1 μM increases the reprogramming efficiency of CB mononuclear cells to induced pluripotent stem cells (iPSCs) by more than 10 times. [2]

Protocol (from reference)

Kinase Assay:

[1]
  • In vitro ROCK assay

    Thiazovivin is dissolved in DMSO. CycLex Rho-kinase assay kit is used to detect ROCK activity using recombinant ROCK in the presence of increasing concentrations of Thiazovivin (~10 μM).
Cell Assay:

[4]
  • Cell lines

    ASZ001 cells

  • Concentrations

    0.5 μM

  • Incubation Time

    72 h

  • Method

    Cells were treated with various concnetrations of thiazovivin.

Customer Product Validation

Data from [Arch Toxicol, 2013, 87(12), 2215-31]

Selleck's Thiazovivin has been cited by 85 publications

Long-term engrafting multilineage hematopoietic cells differentiated from human induced pluripotent stem cells [ Nat Biotechnol, 2024, 10.1038/s41587-024-02360-7] PubMed: 39223325
METTL3/METTL14 maintain human nucleoli integrity by mediating SUV39H1/H2 degradation [ Nat Commun, 2024, 15(1):7186] PubMed: 39169036
HBO1 determines SMAD action in pluripotency and mesendoderm specification [ Nucleic Acids Res, 2024, gkae158] PubMed: 38421638
Generation of human vascularized and chambered cardiac organoids for cardiac disease modelling and drug evaluation [ Cell Prolif, 2024, e13631.] PubMed: 38453465
RNA m6A modification regulates L1 retrotransposons in human spermatogonial stem cell differentiation in vitro and in vivo [ Cell Mol Life Sci, 2024, 81(1):92] PubMed: 38363375
Telomerase is essential for cardiac differentiation and sustained metabolism of human cardiomyocytes [ Cell Mol Life Sci, 2024, 81(1):196] PubMed: 38658440
Feeder-free generation and characterization of endocardial and cardiac valve cells from human pluripotent stem cells [ iScience, 2024, 27(1):108599] PubMed: 38170020
Large-scale generation of IL-12 secreting macrophages from human pluripotent stem cells for cancer therapy [ Mol Ther Methods Clin Dev, 2024, 32(1):101204] PubMed: 38390556
Establishment of a human induced pluripotent stem cell line from a patient with dilated cardiomyopathy [ Stem Cell Res, 2024, 78:103467] PubMed: 38861774
Generation of human induced pluripotent stem cell line MHHi029-A from a male Fabry disease patient carrying c.959A > T mutation [ Stem Cell Res, 2024, 77:103404] PubMed: 38552356

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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