Phenylephrine HCl

Catalog No.S2569 Batch:S256903

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Technical Data

Formula

C9H13NO2.HCl
Molecular Weight 203.67 CAS No. 61-76-7
Solubility (25°C)* In vitro DMSO 41 mg/mL (201.3 mM)
Water 41 mg/mL (201.3 mM)
Ethanol 41 mg/mL (201.3 mM)
In vivo (Add solvents to the product individually and in order)
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O
2.05mg/ml Taking the 1 mL working solution as an example, add 50 μL of 41 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
Clear solution
5% DMSO 95% Corn oil
2.05mg/ml Taking the 1 mL working solution as an example, add 50 μL of 41 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description Phenylephrine HCl is a selective α1-adrenergic receptor agonist, used primarily as a decongestant.
Targets
α1-adrenergic receptor [1]
In vitro

Phenylephrine causes a rapid translocation of PKC-epsilon (EC50 = 0.9 mM) but the proportion lost from the soluble fraction is less than with ET-1. [1] Phenylephrine at pCa 7 causes a dose-dependent increase in contractile force of the hyperpermeable cells, which is reversible on addition of phentolamine. [2] Phenylephrine also protects cardiomyocytes against subsequent 24 h treatment with hypoxia and serum deprivation. Phenylephrine prevents the down-regulation of Bcl-2 and Bcl-X mRNA/protein and induces hypertrophic growth. Phenylephrine-mediated protection is abrogated by the phosphatidylinositol 3-kinase (PI 3-kinase) inhibitor wortmannin and is mimicked by the caspase-9 peptidic inhibitor LEHD-fmk. [3] Phenylephrine stimulates phosphoinositide (PI) hydrolysis, cell growth, and expression of several genes [e.g., atrial natriuretic factor (ANF)] often associated with cardiac hypertrophy. [4] Phenylephrine (1 礛) markedly potentiates HGF-induced hepatocyte DNA synthesis and proliferation. [5] Phenylephrine (10 mM) reversibly increases I(Ca,L) (51.3%; n = 40) and shifted peak I(Ca,L) activation voltage by -10 mV. Phenylephrine also increases local, subsarcolemmal SR Ca2+ release via IP3-dependent signaling. Phenylephrin-induced NOi release requires stimulation of both PI-3K/Akt and IP3-dependent Ca2+ signaling. Phenylephrine-induced NOi release is inhibited by each of 1 mM prazocin, 10 mM L-NIO, 10 mM W-7, 10 mM LY294002, 2 mM H-89, 10 mM ryanodine, 5 mM thapsigargin, 2 mM 2-APB or 10 mM xestospongin C. [6]

Protocol (from reference)

Customer Product Validation

Data from [Data independently produced by , , Stem Cells Dev, 2017, 26(7):528-540]

Selleck's Phenylephrine HCl has been cited by 9 publications

A modified apical resection model with high accuracy and reproducibility in neonatal mouse and rat hearts [ NPJ Regen Med, 2023, 8(1):9] PubMed: 36806296
α1-adrenoceptor stimulation ameliorates lipopolysaccharide-induced lung injury by inhibiting alveolar macrophage inflammatory responses through NF-κB and ERK1/2 pathway in ARDS [ Front Immunol, 2022, 13:1090773] PubMed: 36685596
α1-adrenoceptor stimulation ameliorates lipopolysaccharide-induced lung injury by inhibiting alveolar macrophage inflammatory responses through NF-κB and ERK1/2 pathway in ARDS [ Front Immunol, 2022, 10.3389/fimmu.2022.1090773] PubMed: 36685596
Rap1GAP Mediates Angiotensin II-Induced Cardiomyocyte Hypertrophy by Inhibiting Autophagy and Increasing Oxidative Stress [ Oxid Med Cell Longev, 2021, 2021:7848027] PubMed: 33936386
A CRM1 Inhibitor Alleviates Cardiac Hypertrophy and Increases the Nuclear Distribution of NT-PGC-1α in NRVMs [ Front Pharmacol, 2019, 10:465] PubMed: 31133853
17β-Estradiol nongenomically induces vascular endothelial H2S release by promoting phosphorylation of cystathionine γ-lyase [ J Biol Chem, 2019, 294(43):15577-15592] PubMed: 31439665
The immunoproteasome catalytic β5i subunit regulates cardiac hypertrophy by targeting the autophagy protein ATG5 for degradation. [ Sci Adv, 2019, 5(5):eaau0495] PubMed: 31086810
N‑terminal truncated peroxisome proliferator‑activated receptor‑γ coactivator‑1α alleviates phenylephrine‑induced mitochondrial dysfunction and decreases lipid droplet accumulation in neonatal rat cardiomyocytes. [ Mol Med Rep, 2018, 18(2):2142-2152] PubMed: 29901150
Efficient Differentiation of TBX18+/WT1+ Epicardial-Like Cells from Human Pluripotent Stem Cells Using Small Molecular Compounds. [Zhao J, et al. Stem Cells Dev, 2017, 26(7):528-540] PubMed: 27927069

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.