Mizoribine

Catalog No.S1384 Batch:S138404

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Technical Data

Formula

C9H13N3O6
Molecular Weight 259.22 CAS No. 50924-49-7
Solubility (25°C)* In vitro DMSO 52 mg/mL (200.6 mM)
Water 52 mg/mL (200.6 mM)
Ethanol Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description Mizoribine is an imidazole nucleoside and an immunosuppressant that inhibits HCV RNA replication with IC50 of ~ 100 μM for anti-HCV activity. Mizoribine is a selective inhibitor of inosine-5'-monophosphate dehydrogenase (IMPDH) and guanosine monophosphate synthetase. Mizoribine also inhibits SARS-CoV.
Targets
Monophosphate synthetase [2]
In vitro

Mizoribine (1-50 mg/mL) is able to inhibit T cell proliferation by 10-100% in a dose-dependent fashion to all stimuli tested. Mizoribine leads to a decrease in intracellular GTP levels, and that repletion of GTP reversed its antiproliferative effects. [1] Mizoribine causes a dose-dependent inhibition of T cell proliferation, which is reversible with the addition of 50 mM guanosine to replete guanine ribonucleotide pools. Mizoribine selectively inhibits guanine ribonucleotide formation in purified T cells, whereas the effect of 6-mercaptopurine (6MP) appears to be more dependent on adenine ribonucleotide depletion. [2] Mizoribine inhibits HCV RNA replication with IC50 of 100 μM. [3]
In vivo

Mizoribine (5 or 10 mg/kg) suppresses urinary albumin excretion in the rats. Mizoribine (5 or 10 mg/kg) inhibits the development of glomerulosclerosis, interstitial fibrosis and macrophage infiltration in the kidney in the rats. Mizoribine (5 or 10 mg/kg) increases the expression of MCP-1, OPN and TGF-β1 mRNA in untreated OLETF rats. [4]

Protocol (from reference)

Selleck's Mizoribine has been cited by 6 publications

IDH2 stabilizes HIF-1α-induced metabolic reprogramming and promotes chemoresistance in urothelial cancer [ EMBO J, 2023, e110620.] PubMed: 36637036
SARS-CoV-2 Nsp14 mediates the effects of viral infection on the host cell transcriptome [ bioRxiv, 2022, 2021.07.02.450964] PubMed: 35194610
Stress-induced inflammation evoked by immunogenic cell death is blunted by the IRE1α kinase inhibitor KIRA6 through HSP60 targeting [ Cell Death Differ, 2021, 10.1038/s41418-021-00853-5] PubMed: 34453119
Stress-induced inflammation evoked by immunogenic cell death is blunted by the IRE1α kinase inhibitor KIRA6 through HSP60 targeting [ Cell Death Differ, 2021, 10.1038/s41418-021-00853-5] PubMed: 34453119
IMPDH Inhibitors for Antitumor Therapy in Tuberous Sclerosis Complex [ JCI Insight, 2020, 9;5(7):e135071] PubMed: 32271165
mTORC1 Couples Nucleotide Synthesis to Nucleotide Demand Resulting in a Targetable Metabolic Vulnerability. [ Cancer Cell, 2017, 32(5):624-638] PubMed: 29056426

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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