Menadione (Vitamin K3)

Catalog No.S1949 Batch:S194902

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Technical Data

Formula

C11H8O2
Molecular Weight 172.18 CAS No. 58-27-5
Solubility (25°C)* In vitro DMSO 34 mg/mL (197.46 mM)
Ethanol 34 mg/mL (197.46 mM)
Water Insoluble
In vivo (Add solvents to the product individually and in order)
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O
1.7mg/ml Taking the 1 mL working solution as an example, add 50 μL of 34 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
Clear solution
5% DMSO 95% Corn oil
1.7mg/ml Taking the 1 mL working solution as an example, add 50 μL of 34 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description Menadione (Vitamin K3), a fat-soluble compound, is an inhibitor of Cdc25 phosphatase and mitochondrial DNA polymerase γ (pol γ), used as a nutritional supplement.
Targets
Cdc25 phosphatase [6] DNA polymerase γ [7]
In vitro

Menadione prevents mitochondrial Ca(2+) uptake in normal pancreatic acinar cells, which permits rapid spread of Ca(2+) throughout the cell. Menadione-induced mitochondrial depolarisation is due to induction of the permeability transition pore. [1] Menadione (nontoxic concentration) results in a brief activation of extracellular signal-regulated kinase (ERK) and c-Jun N-terminal kinase (JNK) in the RALA255-10G rat hepatocyte cell line. [2] Menadione is equipotent against multidrug-resistant and parental leukemia cell lines with IC50 values of 13.5 mM and 18 mM, respectively. Menadione inhibits the incorporation of radioactive thymidine, uridine and amino acids into DNA, RNA and protein, respectively, in three humancancer cell lines. [3] Menadione at 1-20 mM dose- and time-dependently inhibits cell proliferation of AR4-2J cells. Menadione (100 mM) causes rapid cell death. Menadione (100 mM) induces DNA smear in electrophoresis indicative of necrosis, while lower concentrations (10-20 mM) induces a DNA ladder indicative of apoptosis. Menadione at 1-20 mM induces wild-type P53, whereas the 100 mM menadione has a minor effect on wild-type P53. [4] Menadione treatment clearly affects the mitochondrial function of Jurkat T cells by inducing a collapse of the inner transmembrane potential (DeltaPsi(m)) and a decrease in inner membrane mass, which could be completely reversed by N-acetylcysteine. [5]

Protocol (from reference)

Selleck's Menadione (Vitamin K3) has been cited by 9 publications

Sex differences in brain tumor glutamine metabolism reveal sex-specific vulnerabilities to treatment [ Med -N Y, 2022, S2666-6340-2200365-8] PubMed: 36108629
SUCLA2-coupled regulation of GLS succinylation and activity counteracts oxidative stress in tumor cells [ Mol Cell, 2021, S1097-2765(21)00269-0] PubMed: 33991485
DNA glycosylase deficiency leads to decreased severity of lupus in the Polb-Y265C mouse model [ DNA Repair (Amst), 2021, 105:103152] PubMed: 34186496
Repurposing FDA approved drugs as radiosensitizers for treating hypoxic prostate cancer [ BMC Urol, 2021, 21(1):96] PubMed: 34210300
Synthetic vitamin K analogs inhibit inflammation by targeting the NLRP3 inflammasome [ Cell Mol Immunol, 2020, 10.1038/s41423-020-00545-z] PubMed: 32917982
Inhibition of Siah2 ubiquitin ligase ameliorates monocrotaline-induced pulmonary arterial remodeling through inactivation of YAP [ Life Sci, 2020, 242:117159] PubMed: 31837334
Inhibition of Siah2 ubiquitin ligase ameliorates monocrotaline-induced pulmonary arterial remodeling through inactivation of YAP. [ Life Sci, 2020, 242:117159] PubMed: 31837334
NQO1 Is a Determinant for Cellular Sensitivity to Anti-Tumor Agent Napabucasin [ Am J Cancer Res, 2020, 1;10(5):1442-1454] PubMed: 32509390
Ubiquitin Modification by the E3 Ligase/ADP-Ribosyltransferase Dtx3L/Parp9. [ Mol Cell, 2017, 66(4):503-516] PubMed: 28525742

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.