Hydroxyurea

Catalog No.S1896 Batch:S189604

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Technical Data

Formula

CH4N2O2
Molecular Weight 76.05 CAS No. 127-07-1
Solubility (25°C)* In vitro DMSO 15 mg/mL (197.23 mM)
Water 15 mg/mL (197.23 mM)
Ethanol Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description Hydroxyurea is an antineoplastic agent that inhibits DNA synthesis through the inhibition of ribonucleoside diphosphate reductase. Hydroxyurea activates apoptosis and autophagy. Hydroxyurea is used to treat HIV infection.
Targets
ribonucleoside diphosphate reductase [1]
In vitro hydroxyurea can inhibit HIV-1 replication. In vitro experiments have shown that the 90% inhibitory concentration (IC90) of hydroxyurea for laboratory strains of HIV-1 in activated PBMC is 0.4 mM. Hydroxyurea was also found to be synergistic with the nucleoside reverse transcriptase inhibitor didanosine and to inhibit HIV-1 replication in activated PBMC; this inhibition may be due to a reduction in deoxynucleoside triphosphate pool sizes. Hydroxyurea has been shown to sensitize didanosine-resistant mutants[1][2].hydroxyurea has demonstrated activity in the treatment of sickle cell anemia by increasing the production of fetal hemoglobin, which reduces hemolysis in patients with this disease. Hydroxyurea exerts its cytostatic effect through inhibition of ribonucleotide reductase—the rate-limiting enzyme responsible for the conversion of ribonucleotides to deoxyribonucleotides, which are essential for DNA synthesis. As a result, cellular division is arrested in the S phase[1].

Protocol (from reference)

Cell Assay:[3]
  • Cell lines

    Erythroid cells

  • Concentrations

    30 μM

  • Incubation Time

    96 h

  • Method

    Erythroid cells obtained from peripheral blood of the same patients(Thirteen β-Thal/HbE patients are treated with hydroxyurea orally for 2 years at a starting dose of 5 mg/kg/day for 5 days/week with escalation to a maximum of 10 mg/kg/day) 1 year after they had stopped hydroxyurea treatment are treated with hydroxyurea in vitro.Treatment of cells performs in primary culture with 30 μM hydroxyurea for 96 hours.
Animal Study:[4]
  • Animal Models

    Female athymic (nu/nu) nude mice(used for xenograft model)

  • Dosages

    1500 mg/kg

  • Administration

    i.p.

Customer Product Validation

Data from [Data independently produced by , , Blood, 2017, 130(26):2848-2859]

Data from [Data independently produced by , , Int J Biochem Cell Biol, 2018, 105:1-12]

Selleck's Hydroxyurea has been cited by 46 publications

TOPORS E3 ligase mediates resistance to hypomethylating agent cytotoxicity in acute myeloid leukemia cells [ Nat Commun, 2024, 15(1):7360] PubMed: 39198401
Necrosulfonamide causes oxidation of PCM1 and impairs ciliogenesis and autophagy [ iScience, 2024, 27(4):109580] PubMed: 38600973
CK2-dependent degradation of CBX3 dictates replication fork stalling and PARP inhibitor sensitivity [ Sci Adv, 2024, 10(21):eadk8908] PubMed: 38781342
CDC5L surveils cellular stress responses and stress granule formation through transcriptional repression [ bioRxiv, 2024, 10.1101/2024.01.04.574133] PubMed: none
Extensive jejunal injury is repaired by migration and transdifferentiation of ileal enterocytes in zebrafish [ Cell Rep, 2023, 42(7):112660] PubMed: 37342912
Targeting MCL-1 triggers DNA damage and an anti-proliferative response independent from apoptosis induction [ Cell Rep, 2023, 42(10):113176] PubMed: 37773750
Lnc956-TRIM28-HSP90B1 complex on replication forks promotes CMG helicase retention to ensure stem cell genomic stability and embryogenesis [ Sci Adv, 2023, 9(4):eadf6277] PubMed: 36706191
Lnc956-TRIM28-HSP90B1 complex on replication forks promotes CMG helicase retention to ensure stem cell genomic stability and embryogenesis [ Sci Adv, 2023, 9(4):eadf6277] PubMed: 36706191
Genetic instability from a single S phase after whole-genome duplication [ Nature, 2022, 604(7904):146-151] PubMed: 35355016
Intercellular transfer of activated STING triggered by RAB22A-mediated non-canonical autophagy promotes antitumor immunity [ Cell Res, 2022, 10.1038/s41422-022-00731-w] PubMed: 36280710

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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