Fulvestrant

Catalog No.S1191 Batch:S119117

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Technical Data

Formula

C32H47F5O3S
Molecular Weight 606.77 CAS No. 129453-61-8
Solubility (25°C)* In vitro DMSO 100 mg/mL (164.8 mM)
Ethanol 100 mg/mL (164.8 mM)
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description Fulvestrant is an estrogen receptor (ER) antagonist with IC50 of 0.94 nM in a cell-free assay. Fulvestrant also induces autophagy and apoptosis and has antitumor activity.
Targets
ER [1]
(Cell-free assay)
0.94 nM
In vitro Fulvestrant is an effective inhibitor of the growth of ER-positive MCF-7 (with IC50 of 0.29 nM) but with no effect on the growth of ER-negative BT-20 human breast cancer cells. Fulvestrant causes accumulation of cells in G0/G1 and also reduces the proportion of cells capable of continued DNA synthesis. [1] Fulvestrant competitively inhibits binding of oestradiol to the estrogen receptor. Fulvestrant blocks nuclear localization of the ER through impairing receptor dimerisation, and energy-dependent nucleo-cytoplasmic shuttling. Because of the instability of fulvestrant-ER complex, the binding of Fulvestrant with ER finally results in accelerated degradation of the ER protein. [2] Fulvestrant (10 nM) not only decreases IGF-IR mRNA levels but also decreases the half-life. [3] Treatment with 100 μM Fulvestrant leads to a time dependent increase of TNFR1 and TRADD steady-state mRNA levels in MCF-7 cells. [4] Fulvestrant is capable of down-regulating androgen receptor expression and diminishes androgenic responses in LNCaP human prostate cancer cells. Fulvestrant also significantly attenuates R1881-stimulated growth by 70%. [5] Fulvestrant is able to modulate mitosis and cell death in immature cerebellar neurons via rapid activation of MAPK. [6]
In vivo Fulvestrant is devoid of uterotropic activity, and when co-administered with estradiol, it effectively blocks the uterotropic action of estradiol with ED50 of 0.06 mg/kg/day s.c. in immature female rats. A single s.c. injection of 5 mg of Fulvestrant suspension blocks completely the growth of MCF-7 xenografts. The growth of transplants of the BrlO human breast tumor is also suppressed effectively by 10 μM Fulvestrant. [1] Fulvestrant (10 mg/rat, s.c.) reduces the androgen receptor expression, ERK1/2 phosphorylation and cell proliferation in the rat ventral prostate. [7] Fulvestrant also displays anti-angiogenesis in the chick egg chorioallantoic membrane. [8]

Protocol (from reference)

Cell Assay:[1]
  • Cell lines

    MCF-7 breast cancer cells

  • Concentrations

    2.9 nM

  • Incubation Time

    5 days

  • Method

    MCF-7 cells are cultured in multiwell plates (24-well, seeding density 4 × 104) in minimal essential medium containing phenol red, insulin (10 μg/mL), and 5% charcoal-stripped fetal calf serum without additional estradiol. Fulvestrant and/or estradiol are added in fresh medium 2 days after seeding. Cultures are maintained for 5 days with one further medium change and growth is assessed by measurement of total cell protein at the beginning and end of treatment and compared with that of controls treated with ethanol (0.1%) alone.
Animal Study:[6]
  • Animal Models

    The human breast cancer xenografts MCF-7 in nude mice

  • Dosages

    5 mg/mouse

  • Administration

    s.c. injection

Customer Product Validation

Data from [Data independently produced by Mol Cancer Ther, 2014, 13(1), 230-8]

Data from [PLoS One, 2011, 6, e21112]

Data from [PLoS One, 2011, 6, e21112]

, , Oncogene, 2017, 36(16):2255-2264

Selleck's Fulvestrant has been cited by 174 publications

EGFR and HER2 hyper-activation mediates resistance to endocrine therapy and CDK4/6 inhibitors in ER+ breast cancer [ Cancer Lett, 2024, 593:216968] PubMed: 38788968
UBE2M forms a positive feedback loop with estrogen receptor to drive breast cancer progression and drug resistance [ Cell Death Dis, 2024, 15(8):590] PubMed: 39138151
Intracellular cartilage oligomeric matrix protein augments breast cancer resistance to chemotherapy [ Cell Death Dis, 2024, 15(7):480] PubMed: 38965233
Methylation of the chromatin modifier KMT2D by SMYD2 contributes to therapeutic response in hormone-dependent breast cancer [ Cell Rep, 2024, 43(5):114174] PubMed: 38700982
Establishing conditions for the generation and maintenance of estrogen receptor-positive organoid models of breast cancer [ Breast Cancer Res, 2024, 26(1):56] PubMed: 38553763
Novel Insight into the mechanism of di (2-ethylhexyl) phthalate (DEHP) impairing early follicle development [ Ecotoxicol Environ Saf, 2024, 285:117043] PubMed: 39293100
Overexpression of COL11A1 confers tamoxifen resistance in breast cancer [ NPJ Breast Cancer, 2024, 10(1):38] PubMed: 38806505
Efficacy of FERscore in predicting sensitivity to ferroptosis inducers in breast cancer [ NPJ Breast Cancer, 2024, 10(1):74] PubMed: 39164282
The combination of breast cancer PDO and mini-PDX platform for drug screening and individualized treatment [ J Cell Mol Med, 2024, 28(9):e18374] PubMed: 38722288
Macrophages Promote Subtype Conversion and Endocrine Resistance in Breast Cancer [ Cancers (Basel), 2024, 16(3)678] PubMed: 38339428

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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