Fisetin

Catalog No.S2298 Batch:S229805

Technical Data

Formula	C₁₅H₁₀O₆
Molecular Weight	286.24	CAS No.	528-48-3
Solubility (25°C)*	In vitro	DMSO	57 mg/mL (199.13 mM)
		Ethanol	5 mg/mL (17.46 mM)
		Water	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description

Fisetin (Fustel) is a potent sirtuin activating compound (STAC) and an agent that modulates sirtuins.

Targets

Sirtuin ^[1]

In vitro

Fisetin selectively kills SPiDER-β-Gal positive senescence neural cells in vitro.^[2]

In vivo

Oral administration of fisetin, a senolytic drug, reduces the number of senescent neural cells observed, the SASP expression level, and depressive behavior in MRL/lpr mice.^[2]

Protocol (from reference)

Cell Assay:^{^[2]}	Cell lines Irradiated senescent Neuro-2a cells and non-irradiated Neuro-2a cells Concentrations 5, 10, or 20 μM Incubation Time 48 h Method Irradiated senescent Neuro-2a cells and non-irradiated Neuro-2a cells are seeded on a 96-well black/clear bottom plate at 40,000 cells and 10,000 cells per well, respectively. After senescence induction for 6 days, 5 μM, 10 μM, or 20 μM fisetin is added and the cells are incubated for 48 h. Cell number and cellular senescence are determined by DAPI staining and SPiDER-β-Gal staining, respectively. Cells are washed twice with PBS, fixed in 4% paraformaldehyde at room temperature for 5 min, and washed twice with PBS. Sections are incubated in 20 M SPiDER-β-gal in solution in McIlvaine buffer (pH 6.0) for 60 min at 37 ℃. After washing of tissue sections, nuclei are stained with DAPI. Cells are observed.
Animal Study:^{^[2]}	Animal Models Female MRL/lpr mice, haplotype-matched female MRL/MPJ mice Dosages 100 mg/kg Administration p.o.

Cell Assay:^{^[2]}

Cell lines
Irradiated senescent Neuro-2a cells and non-irradiated Neuro-2a cells
Concentrations
5, 10, or 20 μM
Incubation Time
48 h
Method
Irradiated senescent Neuro-2a cells and non-irradiated Neuro-2a cells are seeded on a 96-well black/clear bottom plate at 40,000 cells and 10,000 cells per well, respectively. After senescence induction for 6 days, 5 μM, 10 μM, or 20 μM fisetin is added and the cells are incubated for 48 h. Cell number and cellular senescence are determined by DAPI staining and SPiDER-β-Gal staining, respectively. Cells are washed twice with PBS, fixed in 4% paraformaldehyde at room temperature for 5 min, and washed twice with PBS. Sections are incubated in 20 M SPiDER-β-gal in solution in McIlvaine buffer (pH 6.0) for 60 min at 37 ℃. After washing of tissue sections, nuclei are stained with DAPI. Cells are observed.

Animal Study:^{^[2]}

Animal Models
Female MRL/lpr mice, haplotype-matched female MRL/MPJ mice
Dosages
100 mg/kg
Administration
p.o.

References

Customer Product Validation

: Data from [Data independently produced by , , Alcohol Clin Exp Res, 2016, 40(10):2076-2084.]

Selleck's Fisetin has been cited by 40 publications

TAGLN2 induces resistance signature ISGs by activating AKT-YBX1 signal with dual pathways and mediates the IFN-related DNA damage resistance in gastric cancer [ Cell Death Dis, 2024, 15(8):608]	PubMed: 39168971
Senolytic intervention improves cognition, metabolism, and adiposity in female APPNL-F/NL-F mice [ Geroscience, 2024, 10.1007/s11357-024-01308-8]	PubMed: 39120687
Single-cell morphology encodes functional subtypes of senescence in aging human dermal fibroblasts [ bioRxiv, 2024, 2024.05.10.593637]	PubMed: 38798365
Clearance of defective muscle stem cells by senolytics restores myogenesis in myotonic dystrophy type 1 [ Nat Commun, 2023, 14(1):4033]	PubMed: 37468473
Fisetin ameliorates fibrotic kidney disease in mice via inhibiting ACSL4-mediated tubular ferroptosis [ Acta Pharmacol Sin, 2023, 10.1038/s41401-023-01156-w]	PubMed: 37696989
Tubular cell senescence promotes maladaptive kidney repair and chronic kidney disease after cisplatin nephrotoxicity [ JCI Insight, 2023, 8(8)e166643]	PubMed: 36917180
Post-translational modification of CDK1-STAT3 signaling by fisetin suppresses pancreatic cancer stem cell properties [ Cell Biosci, 2023, 13(1):176]	PubMed: 37743465
PPARγ activation by fisetin mitigates vascular smooth muscle cell senescence via the mTORC2-FoxO3a-autophagy signaling pathway [ Biochem Pharmacol, 2023, S0006-2952(23)00485-9]	PubMed: 37890594
Sexual dimorphic metabolic and cognitive responses of C57BL/6 mice to Fisetin or Dasatinib and quercetin cocktail oral treatment [ Geroscience, 2023, 45(5):2835-2850]	PubMed: 37296266
Fisetin, a Natural Polyphenol, Ameliorates Endometriosis Modulating Mast Cells Derived NLRP-3 Inflammasome Pathway and Oxidative Stress [ Int J Mol Sci, 2023, 24(6)5076]	PubMed: 36982152

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SHIPPING AND STORAGE
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