Estradiol

Catalog No.S1709 Batch:S170906

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Technical Data

Formula

C18H24O2
Molecular Weight 272.38 CAS No. 50-28-2
Solubility (25°C)* In vitro DMSO 54 mg/mL (198.25 mM)
Ethanol 54 mg/mL (198.25 mM)
Water Insoluble
In vivo (Add solvents to the product individually and in order)
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O
2.7mg/ml Taking the 1 mL working solution as an example, add 50 μL of clarified DMSO stock solution of 54 mg/ml to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify it; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description Estradiol (17β-estradiol, β-Estradiol, E2, 17β-Oestradiol) is a human sex hormone and steroid, and the primary female sex hormone. Estradiol upregulates IL-6 expression through the estrogen receptor β (ERβ) pathway.
Targets
Estrogen receptor [1]
In vitro

Estradiol induces new dendritic spines and synapses on hippocampal CA1 pyramidal cells. Estradiol treatment resulted in a 46% increase in NMDA receptor binding. Estradiol treatment increases NMDA receptor binding in parallel with dendritic spine and synapse density. Estradiol treatment results in increased sensitivity of CA1 pyramidal cells to NMDA receptor-mediated synaptic input and that this increase is well correlated with the estradiol-induced increase in dendritic spine density in the apical dendritic tree of these cells. [1]

17 beta-estradiol is found to reduce Ba2+ entry reversibly via Ca2+ channels in acutely dissociated and cultured neostriatal neurons. 17 alpha-Estradiol also reduces Ba2+ currents but is significantly less effective than 17 beta-estradiol in rat neostriatal neurons. [2]

17 beta-estradiol exerts a dose-dependent inhibition of IL-1-, TNF-, and IL-1 and TNF-induced production of bioassayable IL-6. Estradiol inhibits both TNF-induced IL-6 production and osteoclast development in primary bone cell cultures derived from neonatal murine calvaria. [3]
In vivo

Estradiol mediates fluctuation in hippocampal synapse density during the estrous cycle in the adult rat. [4]

Estradiol alone can reverse the ovariectomy-induced decrease in spine density. Estradiol combined with Progesterone initially increases spine density for a period of 2 to 6 hours but then results in a much sharper decrease than is observed following estradiol alone. [5]

Protocol (from reference)

Cell Assay:

[6]
  • Cell lines

    HCC1806 cells

  • Concentrations

    10 nM

  • Incubation Time

    24 h

  • Method

    Cells were treated with vehicle or 10 nM E2 for 24 hours.
Animal Study:

[5]
  • Animal Models

    Female Sprague-Dawley rats

  • Dosages

    10 µg

  • Administration

    s.c.

Selleck's Estradiol has been cited by 23 publications

YAP-mediated GPER signaling impedes proliferation and survival of prostate epithelium in benign prostatic hyperplasia [ iScience, 2024, 27(3):109125] PubMed: 38420594
Testosterone and estradiol reduce inflammation of human macrophages induced by anti-SARS-CoV-2 IgG [ Eur J Immunol, 2024, e2451226.] PubMed: 39246165
Synthesis of a [18F]F Estradiol Derivative via Click Chemistry Using an Automated Synthesis Module: In Vitro Evaluation as Potential Radiopharmaceutical for Breast Cancer Imaging [ Pharmaceuticals (Basel), 2024, 17(3)388] PubMed: 38543174
Injectable Biomimetic Hydrogel Constructs for Cell-Based Menopausal Hormone Therapy with Reduced Breast Cancer Potential [ Biomater Res, 2024, 28:0054] PubMed: 39135549
Novel Estrogen Receptor Dimerization BRET-Based Biosensors for Screening Estrogenic Endocrine-Disrupting Chemicals [ Biomater Res, 2024, 28:0010] PubMed: 38464469
Drug-drug interaction potentials of tucatinib inhibition of human UDP-glucuronosyltransferases [ Chem Biol Interact, 2023, 381:110574] PubMed: 37263554
Alternative promoters and splicing create multiple functionally distinct isoforms of oestrogen receptor alpha in breast cancer and healthy tissues [ Cancer Med, 2023, 10.1002/cam4.6508] PubMed: 37676103
DNMT3B-mediated FAM111B methylation promotes papillary thyroid tumor glycolysis, growth and metastasis [ Int J Biol Sci, 2022, 18(11):4372-4387] PubMed: 35864964
Multiple PDE3A modulators act as molecular glues promoting PDE3A-SLFN12 interaction and induce SLFN12 dephosphorylation and cell death [ Cell Chem Biol, 2022, S2451-9456(22)00006-X] PubMed: 35104454
Estrogens revert neutrophil hyperplasia by inhibiting Hif1α-cMyb pathway in zebrafish myelodysplastic syndromes models [ Cell Death Discov, 2022, 8(1):323] PubMed: 35842445

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.