DHA (Dihydroartemisinin)

Catalog No.S2290 Batch:S229009

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Technical Data

Formula

C15H24O5
Molecular Weight 284.35 CAS No. 71939-50-9
Solubility (25°C)* In vitro DMSO 50 mg/mL (175.83 mM)
Ethanol 7 mg/mL (24.61 mM)
Water Insoluble
In vivo (Add solvents to the product individually and in order)
Clear solution
6%DMSO 94%Corn oil
3.0mg/ml Taking the 1 mL working solution as an example, add 60 μL of 50 mg/ml clear DMSO stock solution to 940 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results. 
10% DMSO 40% PEG 300 5%Tween80  45%H2O
4.0mg/ml
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description DHA (Dihydroartemisinin) is a semi-synthetic derivative of artemisinin and isolated from the traditional Chinese herb Artemisia annua. Dihydroartemisinin induces autophagy and apoptosis by suppressing NF-κB activation.
Targets
NF-κB [4]
In vitro

Dihydroartemisinin (DHA) inhibits the growth of certain cancer cell lines and xenograft tumors such as leukemia, glioma, fibrosarcoma, and breast, cervical, ovarian, lung, oral and pancreatic cancer. DHA inhibits cell and tumor growth by modulating various tumor-suppressive pathways, such as inhibiting cell proliferation and inducing apoptosis through regulation of proliferation- and apoptosis-related proteins.DHA inhibits the proliferation and viability of cells in a dose-dependent manner and induces apoptosis.DHA-mediated cytotoxicity is tumor selective. The endoperoxide bridge of DHA is reportedly essential for its cytotoxicity because it reacts with intracellular ferrous iron to generate reactive oxygen species or carbon-centered radicals, leading to cytotoxicity[1].
In vivo

DHA significantly inhibited HCC cell growth in vitro and in vivo via inducing G2/M cell cycle arrest and apoptosis[2].

DHA has been shown in the rat whole embryo culture (WEC) to primarily affect primitive red blood cells (RBCs) causing subsequent tissue damage and dysmorphogenesis[3].

Protocol (from reference)

Cell Assay:

[1]
  • Cell lines

    pancreatic cancer cell line BxPc3-RFP

  • Concentrations

    2.5, 10, 40, or 80 μM

  • Incubation Time

    24, 48, and 72 h

  • Method

    BxPc3-RFP cells (3.5×104cells/well) were seeded in poly D-lysine-coated black, μClear 96-well plates with 0.2 ml medium. After 24 h, the cells were treated with dimethyl sulfoxide (DMSO) (control) or different concentrations (2.5, 10, 40, or 80 μM) of DHA dissolved in DMSO for 24, 48, and 72 h. At each time point, the fluorescence intensity emitted from cells was measured.
Animal Study:

[2]
  • Animal Models

    females, BALB/cA Jcl-nu/nu mice

  • Dosages

    25 mg/kg 

  • Administration

    i.p.

Customer Product Validation

, , Oncotarget, 2015, 6(7):5275-91.

, , J Exp Clin Cancer Res, 2017, 36(1):68

, , PLoS One, 2015, 10(3):e0120426.

Data from [Data independently produced by , , Biochem Pharmacol, 2018, 150:72-85]

Selleck's DHA (Dihydroartemisinin) has been cited by 34 publications

Dihydroartemisinin remodels tumor micro-environment and improves cancer immunotherapy through inhibiting cyclin-dependent kinases [ Int Immunopharmacol, 2024, 139:112637] PubMed: 39033659
Network-based prediction of anti-cancer drug combinations [ Front Pharmacol, 2024, 15:1418902] PubMed: 39211773
A pH Fingerprint Assay to Identify Inhibitors of Multiple Validated and Potential Antimalarial Drug Targets [ ACS Infect Dis, 2024, 10.1021/acsinfecdis.3c00588] PubMed: 38499199
Dihydroartemisinin, a potential PTGS1 inhibitor, potentiated cisplatin-induced cell death in non-small cell lung cancer through activating ROS-mediated multiple signaling pathways [ Neoplasia, 2024, 51:100991] PubMed: 38507887
Resistance to FOXM1 inhibitors in breast cancer is accompanied by impeding ferroptosis and apoptotic cell death [ Breast Cancer Res Treat, 2024, 10.1007/s10549-024-07420-9] PubMed: 38980505
Protocol for analysis of intracellular conversion of artezomib molecules into new proteasome inhibitors in Plasmodium falciparum parasites [ STAR Protoc, 2024, 5(1):102896] PubMed: 38363687
Circulating extracellular vesicles are monitoring biomarkers of anti-PD1 response and enhancer of tumor progression and immunosuppression in metastatic melanoma [ J Exp Clin Cancer Res, 2023, 42(1):251] PubMed: 37759291
Micromolar Dihydroartemisinin Concentrations Elicit Lipoperoxidation in Plasmodium falciparum-Infected Erythrocytes [ Antioxidants (Basel), 2023, 12(7)1468] PubMed: 37508006
Dihydroartemisinin-induced ferroptosis in acute myeloid leukemia: links to iron metabolism and metallothionein [ Cell Death Discov, 2023, 9(1):97] PubMed: 36928207
Dihydroartemisinin-induced ferroptosis in acute myeloid leukemia: links to iron metabolism and metallothionein [ Cell Death Discov, 2023, 9(1):97] PubMed: 36928207

RETURN POLICY
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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.