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Technical Data
| Formula | C11H12Cl2N2O5 |
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| Molecular Weight | 323.13 | CAS No. | 56-75-7 | |
| Solubility (25°C)* | In vitro | DMSO | 65 mg/mL (201.15 mM) | |
| Ethanol | 65 mg/mL (201.15 mM) | |||
| Water | Insoluble | |||
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* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.) |
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Biological Activity
| Description | Chloramphenicol (Chloromycetin) is a bacteriostatic by inhibiting protein synthesis. |
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| In vitro | Chloramphenicol results in a constitutive increase in the level of cspA transcript and constitutive production of CS7.4. [1] Chloramphenicol down-regulates the mitochondrial DNA-encoded Cox I protein, but not nuclear-encoded proteins. Chloramphenicol increases cellular levels of the p21(waf1/cip1) protein and p21(waf1/cip1) mRNA through a p53-independent pathway. [2] Chloramphenicol, at concentrations greater than 0.1 g/L (0.3 mM), inhibits the denitrifying enzyme activity (DEA) of slurries of humisol and sandy loam soils by disrupting the activity of existing nitrate reductase enzymes. [3] Chloramphenicol, at concentrations from 5 mM to 2 mM initiates apoptosis in dividing cells from a monkey kidney-derived cell line and in haematopoietic progenitor cells from human neonatal cord blood. Chloramphenicol suppresses growth of progenitor cells. [4] |
| In vivo | Chloramphenicol results in increased t1/2(beta) (by 209%), and decreased ClB (by 45%), and prolonged recovery indices (by 768 to 946%) in greyhounds. [5] Chloramphenicol suppresses increases in the body weight and liver weight of experimental animals and at the same time induced a remarkable increase in lipid peroxidation in the liver during the formation of megamitochondria. Chloramphenicol-induced formation of megamitochondria is not simply ascribed to the suppression of the dividing process of mitochondria due to lowered protein synthesis in mitochondria but is intimately related to oxidative stress. [6] |
Protocol (from reference)
References
Selleck's Chloramphenicol has been cited by 3 publications
| The mitochondrial protein ERAL1 suppresses RNA virus infection by facilitating RIG-I-like receptor signaling [ Cell Rep, 2021, 34(3):108631] | PubMed: 33472079 |
| The mTORC1-eIF4F axis controls paused pluripotency [ EMBO Rep, 2021, e53081] | PubMed: 34866316 |
| Twenty-seven-nucleotide repeat insertion in the rplV gene confers specific resistance to macrolide antibiotics in Staphylococcus aureus [ Oncotarget, 2018, 9(40):26086-26095] | PubMed: 29899844 |
RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.
SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.
NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.