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Formula | C55H85N17O25S4 |
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Molecular Weight | 1512.62 | CAS No. | 9041-93-4 | |
Solubility (25°C)* | In vitro | DMSO | 100 mg/mL (66.11 mM) | |
Water | 100 mg/mL (66.11 mM) | |||
Ethanol | Insoluble | |||
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.) |
Description | Bleomycin sulfate is a glycopeptide antibiotic and a DNA synthesis inhibitor, as well as an anticancer agent for squamous cell carcinomas (SCC) with an IC50 of 4 nM in UT-SCC-19A cells. Bleomycin sulfate is a DNA synthesis inhibitor. |
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In vitro | UT-SCC-12A and UT-SCC-12B are both more resistant to Bleomycin sulfate with IC50 of 14.2 nM and 13 nM, respectively. [1] Alveolar macrophages incubated with 0.01 μg/mL to 1μg /mL Bleomycin sulfate for 18 hours secretes significantly more fibroblast growth factor than macrophages incubated without Bleomycin sulfate. Macrophages stimulated with Bleomycin sulfate continues to produce significant amounts of fibroblast growth factor even after Bleomycin sulfate is removed and replaced with fresh (Bleomycin sulfate-free) media. Fibroblast growth factor secretion by Bleomycin sulfate-stimulated alveolar macrophages is inhibited by cycloheximide, and the 5-lipoxygenase inhibitors NDGA (nordihydroguaiaretic acid) and BW755c, indicating not only a requirement for protein synthesis but also for metabolites of the 5-lipoxygenase pathway of arachidonic acid metabolism for full expression of activity. [2] Bleomycin sulfate (400 µg/mL) incubation for 24 hours decreases the viability of NTera-2 cells, and increases caspase-3, -8 and -9 activities, Bax and cytoplasmic cytochrome c levels and decreases Bcl-2 levels. [3] In terms of unstable aberrations, the clastogenic effect of Bleomycin sulfate on ADIPO-P2 cells persists for at least 10 days after exposure. Bleomycin sulfate-induced telomere instability in mammalian cells persists for several generations after exposure. Moreover, the appearance of telomere fusions in Bleomycin sulfate-exposed cells 10 days after treatment suggests that Bleomycin sulfate can induce delayed telomere instability. [4] |
In vivo | Day 7 post-Bleomycin sulfate, CD45+ cells in BALf in NOX-/- is 1.7-fold > WT, 57% of which are Mf that decreases by 67% in WT and 83% in NOX-/- by Day 21. [5] |
Cell Assay: |
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Animal Study: |
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Data from [Data independently produced by AACRElizabeth williamson from university of florida. , 2011]
Profibrogenic macrophage-targeted delivery of mitochondrial protector via exosome formula for alleviating pulmonary fibrosis [ Bioact Mater, 2024, 32:488-501] | PubMed: 37965241 |
Pro-ferroptotic signaling promotes arterial aging via vascular smooth muscle cell senescence [ Nat Commun, 2024, 15(1):1429] | PubMed: 38365899 |
YTHDC1 delays cellular senescence and pulmonary fibrosis by activating ATR in an m6A-independent manner [ EMBO J, 2024, 43(1):61-86] | PubMed: 38177310 |
Endothelial H2S-AMPK dysfunction upregulates the angiocrine factor PAI-1 and contributes to lung fibrosis [ Redox Biol, 2024, 70:103038] | PubMed: 38266576 |
Circular RNA MKLN1 promotes epithelial-mesenchymal transition in pulmonary fibrosis by regulating the miR-26a/b-5p/CDK8 axis in human alveolar epithelial cells and mice models [ Arch Toxicol, 2024, 98(5):1399-1413] | PubMed: 38460002 |
Calpain-1 Up-Regulation Promotes Bleomycin-Induced Pulmonary Fibrosis by Activating Ferroptosis [ Am J Pathol, 2024, S0002-9440(24)00356-0] | PubMed: 39326733 |
Bleomycin induces senescence and repression of DNA repair via downregulation of Rad51 [ Mol Med, 2024, 30(1):54] | PubMed: 38649802 |
Processing of angiocrine alarmin IL-1α in endothelial cells promotes lung and liver fibrosis [ Int Immunopharmacol, 2024, 134:112176] | PubMed: 38723369 |
Tuberostemonine may enhance the function of the SLC7A11/glutamate antiporter to restrain the ferroptosis to alleviate pulmonary fibrosis [ J Ethnopharmacol, 2024, 318(Pt B):116983] | PubMed: 37532076 |
Loss of ZNF451 mediates fibroblast activation and promotes lung fibrosis [ Respir Res, 2024, 25(1):160] | PubMed: 38600524 |
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