Berberine chloride

Catalog No.S2271 Batch:S227103

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Technical Data

Formula

C20H18NO4.Cl
Molecular Weight 371.81 CAS No. 633-65-8
Solubility (25°C)* In vitro DMSO 38 mg/mL (102.2 mM)
Water Insoluble
Ethanol Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description Berberine chloride is a quaternary ammonium salt from the group of isoquinoline alkaloids. Berberine activates caspase 3 and caspase 8, cleavage of poly ADP-ribose polymerase (PARP) and the release of cytochrome c. Berberine chloride decreases the expression of c-IAP1, Bcl-2 and Bcl-XL. Berberine chloride induces apoptosis with sustained phosphorylation of JNK and p38 MAPK, as well as generation of the ROS. Berberine chloride is a dual topoisomerase I and II inhibitor. Berberine chloride is also a potential autophagy modulator.
Targets
Caspase-3 [1] Caspase-8 [1] PARP [1] cytochrome c [1] cIAP1 [1] View More
In vitro

Compared with regorafenib alone, the combined treatment of Berberine (BBR) and regorafenib significantly inhibits the proliferation of hepatocellular carcinoma (HCC) cells and induces cellular apoptosis.[3]
In vivo

The combined treatment group with Berberine (BBR) and regorafenib has a dramatic inhibitory effect on the growth of hepatocellular carcinoma (HCC) xenograft tumors in nude mice. The increased apoptosis of xenograft tumors is seen in the combined treatment group.[3]

Protocol (from reference)

Cell Assay:

[3]
  • Cell lines

    hepatocellular carcinoma (HCC) cells

  • Concentrations

    5, 10, 20, 40, 80, 160, 320, 640 and 1280 μM

  • Incubation Time

    48 h

  • Method

    HCC cells are seeded into a 96-well plate with 5,000 cells per well. After overnight incubation, HCC cells are treated with the indicated concentrations of regorafenib, Berberine (BBR) or their combination for 24h or 48h. The viability of HCC is examined by MTS Assay and Synergy H1/Epoch microplate reader.The interaction between regorafenib and BBR is quantified by Com-puSyn software. The Cell-LightTM EdU ApolloR567 In Vitro Imaging Kit is used to detect the level of cell proliferation. The Annexin V-FITC/PI Apoptosis Detection Kit and DeadEndTM Fluorometric Tunel System assay are used to measure cellular apoptotic level.
Animal Study:

[3]
  • Animal Models

    Male BALB/c nude mice

  • Dosages

    10mg/kg/day

  • Administration

    i.p.

    (Data sourced from selleck products)

Selleck's Berberine chloride has been cited by 9 publications

Repurposing FDA-Approved Drugs for Temozolomide-Resistant IDH1 Mutant Glioma Using High-Throughput Miniaturized Screening on Droplet Microarray Chip [ Adv Healthc Mater, 2023, e2300591.] PubMed: 37162029
Connexin43 is associated with the progression of clear cell renal carcinoma and is regulated by tangeretin to sygergize with tyrosine kinase inhibitors [ Transl Oncol, 2023, 35:101712] PubMed: 37354638
Berberine chloride (dual topoisomerase I and II inhibitor) modulate mitochondrial uncoupling protein (UCP1) in molecular docking and dynamic with in-vitro cytotoxic and mitochondrial ATP production [ J Biomol Struct Dyn, 2022, 1-11] PubMed: 35612892
Berberine Sensitizes Human Hepatoma Cells to Regorafenib via Modulating Expression of Circular RNAs [ Front Pharmacol, 2021, 12:632201] PubMed: 34220494
Divergent and Overlapping Roles for Selected Phytochemicals in the Regulation of Pathological Cardiac Hypertrophy [ Molecules, 2021, 26(5)1210] PubMed: 33668293
Quantitative Proteomics Analysis of Berberine-Treated Colon Cancer Cells Reveals Potential Therapy Targets [ Biology (Basel), 2021, 10(3)250] PubMed: 33806918
Suppression effect of plant-derived berberine on cyprinid herpesvirus 2 proliferation and its pharmacokinetics in Crucian carp (Carassius auratus gibelio) [ Antiviral Res, 2020, 186:105000] PubMed: 33359191
Berberine, a natural plant alkaloid, synergistically sensitizes human liver cancer cells to sorafenib. [ Oncol Rep, 2018, 40(3):1525-1532] PubMed: 30015938
Characterization of glycosaminoglycan interaction sites and exosite inhibitors of cathepsin K [Haoran Cui, et al. Shandong University, 2014, Haoran Cui]

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.