Artesunate

Catalog No.S2265 Batch:S226504

Technical Data

Formula

C₁₉H₂₈O₈

Molecular Weight

384.42

CAS No.

88495-63-0

Solubility (25°C)*

In vitro

DMSO

77 mg/mL (200.3 mM)

Ethanol

77 mg/mL (200.3 mM)

Water

Insoluble

In vivo (Add solvents to the product individually and in order)

Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O	3.85mg/ml	Taking the 1 mL working solution as an example, add 50 μL of 77 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil	3.85mg/ml	Taking the 1 mL working solution as an example, add 50 μL of 77 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description

Artesunate is a part of the artemisinin group of agents with an IC50 of < 5 μM for small cell lung carcinoma cell line H69. It is a potential inhibitor of STAT-3 and exhibits selective cytotoxicity of cancer cells over normal cells in vitro; A potent inhibitor of EXP1.

Targets

EXP1(a membrane glutathione S-transferase) ^[4]

STAT3 ^[7]

In vitro

ART could alter the biomechanical properties of the glioma cells to inhibit cell proliferation, migration and invasion ^[2]. The ART significantly suppresses the cell proliferation, induces the apoptosis and promoted cell cycle arrest at G0/G1 phase in SKM-1 cells. The mechanisms of ART anti-MDS is associated with the increase of intracellular calciumion concentration and ROS levels. In addition, the pro-apoptotic activity of ART may be involved in the regulation of BCL-2 /BAX ratio and the expressions of P-bad and survivin^[3]. ART treatment demethylates CDH1, which, in turn recovers the E-cadherin activation in SKM-1 cells^[1].

In vivo

Artesunate is a medication used to treat malaria. Artesunate can induce radiosensitivity of HeLa cells in vivo using Xenograft model of nude mice^[5]. Artesunate had a relatively high immunosuppressive activity with low toxicity, and could inhibit T lymphocyte proliferation induced by mitogen and alloantigen^[6].

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines SKM-1 cells Concentrations 0, 12.5, 25, 50 µg/mL Incubation Time 0, 24, 48, and 72 h Method Growth inhibition assay: The SKM-1 cells (1×105/mL) are firstly seeded in 96-well plates. Artesunate is diluted in 0.1% dimethyl sulfoxide (DMSO) producing 0, 12.5, 25, 50µg/mL concentrations and added to the SKM-1 cells with 100 µl per well. A negative control is treated with 0.1% DMSO. At 0, 24, 48, and 72 hours, same amount of MTT solution is added into each well and cultured for extra 4 hours. MTT treated cells are fixed with 150 µL DMSO for 30 min at room temperature and then determined with Evolution 201 and 220 UV-Vis spectrophotometer system at 540 nm.
Animal Study:^{^[5]}	Animal Models BALB/c mice Dosages 100 mg/kg/day Administration i.p.

Cell Assay:^{^[1]}

Cell lines
SKM-1 cells
Concentrations
0, 12.5, 25, 50 µg/mL
Incubation Time
0, 24, 48, and 72 h
Method
Growth inhibition assay: The SKM-1 cells (1×105/mL) are firstly seeded in 96-well plates. Artesunate is diluted in 0.1% dimethyl sulfoxide (DMSO) producing 0, 12.5, 25, 50µg/mL concentrations and added to the SKM-1 cells with 100 µl per well. A negative control is treated with 0.1% DMSO. At 0, 24, 48, and 72 hours, same amount of MTT solution is added into each well and cultured for extra 4 hours. MTT treated cells are fixed with 150 µL DMSO for 30 min at room temperature and then determined with Evolution 201 and 220 UV-Vis spectrophotometer system at 540 nm.

Animal Study:^{^[5]}

Animal Models
BALB/c mice
Dosages
100 mg/kg/day
Administration
i.p.

References

+ Expand

Selleck's Artesunate has been cited by 17 publications

In vitro assessment of the anti-adenoviral activity of artemisinin and its derivatives [ Virus Res, 2024, 349:199448]	PubMed: 39127240
Artesunate ameliorates irinotecan-induced intestinal injury by suppressing cellular senescence and significantly enhances anti-tumor activity [ Int Immunopharmacol, 2023, 119:110205]	PubMed: 37104917
The Antimalarial Drug Artesunate Mediates Selective Cytotoxicity by Upregulating HO-1 in Melanoma Cells [ Biomedicines, 2023, 11(9)2393]	PubMed: 37760834
Artesunate alleviates 5-fluorouracil-induced intestinal damage by suppressing cellular senescence and enhances its antitumor activity [ Discov Oncol, 2023, 14(1):139]	PubMed: 37498338
Artesunate alleviates 5-fluorouracil-induced intestinal damage by suppressing cellular senescence and enhances its antitumor activity [ Discov Oncol, 2023, 14(1):139]	PubMed: 37498338
Artesunate promoted anti-tumor immunity and overcame EGFR-TKI resistance in non-small-cell lung cancer by enhancing oncogenic TAZ degradation [ Biomed Pharmacother, 2022, 155:113705]	PubMed: 36271541
Involvement of Bid in the crosstalk between ferroptotic agent-induced ER stress and TRAIL-induced apoptosis [ J Cell Physiol, 2022, 10.1002/jcp.30863]	PubMed: 35994698
Artesunate Regulates Neurite Outgrowth Inhibitor Protein B Receptor to Overcome Resistance to Sorafenib in Hepatocellular Carcinoma Cells [ Front Pharmacol, 2021, 12:615889]	PubMed: 33716742
In vitro efficacy of artemisinin-based treatments against SARS-CoV-2 [ Sci Rep, 2021, 11(1):14571]	PubMed: 34272426
Artesunate combined with verteporfin inhibits uveal melanoma by regulation of the MALAT1/yes-associated protein signaling pathway [ Oncol Lett, 2021, 22(2):597]	PubMed: 34188699

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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