5-methoxyflavone

Catalog No.S4782 Batch:S478201

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Technical Data

Formula

C16H12O3
Molecular Weight 252.26 CAS No. 42079-78-7
Solubility (25°C)* In vitro DMSO 50 mg/mL (198.2 mM)
 
 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description 5-methoxyflavone is a novel DNA polymerase-beta inhibitor and neuroprotective agent against beta-amyloid toxicity.
Targets
DNA polymerase β [1]
In vitro The treatment with 5-methoxyflavone (5-MF） at various times (3, 6, 12, 18 and 24 h) in MOLT-4 cells results in a time dependent reduction in the levels of antiapoptotic proteins cFLIP, Mcl-1 and an increase in the proapoptotic protein BAX. 5-MF induces both TRAIL-R1(DR4) and TRAIL-R2 (DR5) in a time-dependent manner[2].

Protocol (from reference)

Cell Assay:[2]
  • Cell lines

    human leukemic MOLT-4, U937 cell lines and PBMCs

  • Concentrations

    0-100 μg/ml

  • Incubation Time

    24 h

  • Method

    MOLT-4 or U937 or PBMC cells are treated with DMF or TMF or PMF, 5-MF or 2'-MF at various concentrations for 24 h, then the cell viability is determined by the MTT assay.

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.