4-PBA (4-Phenylbutyric acid)

Catalog No.S3592 Batch:S359204

Technical Data

Formula	C₁₀H₁₂O₂
Molecular Weight	164.20	CAS No.	1821-12-1
Solubility (25°C)*	In vitro	DMSO	33 mg/mL (200.97 mM)
		Ethanol	33 mg/mL (200.97 mM)
		Water	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description

4-PBA (4-Phenylbutyric acid) is a histone deacetylase (HDAC) inhibitor and a key epigenetic inducer of anti-HCV hepatic hepcidin. 4-Phenylbutyric acid inhibits LPS-induced inflammation through regulating endoplasmic-reticulum (ER) stress and autophagy in acute lung injury models.

Targets

HDAC ^[1]

In vitro

4-PBA inhibits the ER stress induced by SiNPs in RAW264.7 cells, as evidenced by the expansion and degranulation of ER, as well as greatly up-regulated Bip and CHOP expressions.^[3]

In vivo

4-PBA attenuates LPS-induced bone loss in lipopolysaccharide (LPS)-treated mice, by increasing area of TRAP-positive osteoclasts (OCs) and serum level of collagen type I fragments.^[4]

Protocol (from reference)

Cell Assay:^{^[3]}	Cell lines Mouse macrophage cell line, RAW264.7 cells Concentrations 3 mM Incubation Time 6 h Method Mouse macrophage cell line, RAW264.7 cells are cultured in DMEM with 10% fetal bovine serum at 37 ℃ in a 5% CO₂ incubator. SiNPs are diluted by DMEM to appropriate concentrations, and an ER stress inhibitor, 4-phenylbutyric acid (4-PBA) is applied (3 mM, 6 h). The SiNPs-treated group is at a concentration of 50 μg/ml in vitro experiments. Similarly, the application of 4-PBA is set up through MTT assay, after SiNPs with or without 4-PBA treatment, cells are harvested for the following measurement. The levels of Bip and CHOP induced by SiNPs are measured.

Cell Assay:^{^[3]}

Cell lines
Mouse macrophage cell line, RAW264.7 cells
Concentrations
3 mM
Incubation Time
6 h
Method
Mouse macrophage cell line, RAW264.7 cells are cultured in DMEM with 10% fetal bovine serum at 37 ℃ in a 5% CO₂ incubator. SiNPs are diluted by DMEM to appropriate concentrations, and an ER stress inhibitor, 4-phenylbutyric acid (4-PBA) is applied (3 mM, 6 h). The SiNPs-treated group is at a concentration of 50 μg/ml in vitro experiments. Similarly, the application of 4-PBA is set up through MTT assay, after SiNPs with or without 4-PBA treatment, cells are harvested for the following measurement. The levels of Bip and CHOP induced by SiNPs are measured.

References

[4] Park HJ, et al. Biochem Pharmacol. 2018 May;151:9-17.

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Selleck's 4-PBA (4-Phenylbutyric acid) has been cited by 17 publications

A novel CSN5/CRT O-GlcNAc/ER stress regulatory axis in platinum resistance of epithelial ovarian cancer [ Int J Biol Sci, 2024, 20(4):1279-1296]	PubMed: 38385070
Melatonin protects RPE cells from necroptosis and NLRP3 activation via promoting SERCA2-related intracellular Ca2+ homeostasis [ Phytomedicine, 2024, 135:156088]	PubMed: 39341129
Inhibition of OGFOD1 by FG4592 confers neuroprotection by activating unfolded protein response and autophagy after ischemic stroke [ J Transl Med, 2024, 22(1):248]	PubMed: 38454480
IRE1α arm of unfolded protein response in muscle-specific TGF-β signaling-mediated regulation of muscle cell immunological properties [ Cell Mol Biol Lett, 2023, 28(1):15]	PubMed: 36849929
Porcine reproductive and respiratory syndrome virus infection triggers autophagy via ER stress-induced calcium signaling to facilitate virus replication [ PLoS Pathog, 2023, 19(3):e1011295]	PubMed: 36972295
Routes of Albumin Overload Toxicity in Renal Tubular Epithelial Cells [ Int J Mol Sci, 2023, 24(11)9640]	PubMed: 37298591
Routes of Albumin Overload Toxicity in Renal Tubular Epithelial Cells [ Int J Mol Sci, 2023, 24(11)9640]	PubMed: 37298591
Large HBV Surface Protein-Induced Unfolded Protein Response Dynamically Regulates p27 Degradation in Hepatocellular Carcinoma Progression [ Int J Mol Sci, 2023, 10.3390/ijms241813825]	PubMed: 37762128
Endoplasmic reticulum stress mediates environmental particle-induced inflammatory response in bronchial epithelium [ J Immunotoxicol, 2023, 10.1080/1547691X.2023.2229428]	PubMed: 37417782
Effects of endoplasmic reticulum stress on erectile function in rats with cavernous nerve injury [ Sex Med, 2023, 11(4):qfad050]	PubMed: 37674767

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