4-PBA (4-Phenylbutyric acid)

Catalog No.S3592 Batch:S359202

Technical Data

Formula	C₁₀H₁₂O₂
Molecular Weight	164.20	CAS No.	1821-12-1
Solubility (25°C)*	In vitro	DMSO	500 mg/mL (3045.06 mM)
		Ethanol	33 mg/mL (200.97 mM)
		Water	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description

4-PBA (4-Phenylbutyric acid) is a histone deacetylase (HDAC) inhibitor and a key epigenetic inducer of anti-HCV hepatic hepcidin. 4-Phenylbutyric acid inhibits LPS-induced inflammation through regulating endoplasmic-reticulum (ER) stress and autophagy in acute lung injury models.

Targets

HDAC ^[1]

In vitro

4-PBA inhibits the ER stress induced by SiNPs in RAW264.7 cells, as evidenced by the expansion and degranulation of ER, as well as greatly up-regulated Bip and CHOP expressions.^[3]

In vivo

4-PBA attenuates LPS-induced bone loss in lipopolysaccharide (LPS)-treated mice, by increasing area of TRAP-positive osteoclasts (OCs) and serum level of collagen type I fragments.^[4]

Protocol (from reference)

Cell Assay:^{^[3]}	Cell lines Mouse macrophage cell line, RAW264.7 cells Concentrations 3 mM Incubation Time 6 h Method Mouse macrophage cell line, RAW264.7 cells are cultured in DMEM with 10% fetal bovine serum at 37 ℃ in a 5% CO₂ incubator. SiNPs are diluted by DMEM to appropriate concentrations, and an ER stress inhibitor, 4-phenylbutyric acid (4-PBA) is applied (3 mM, 6 h). The SiNPs-treated group is at a concentration of 50 μg/ml in vitro experiments. Similarly, the application of 4-PBA is set up through MTT assay, after SiNPs with or without 4-PBA treatment, cells are harvested for the following measurement. The levels of Bip and CHOP induced by SiNPs are measured.

Cell Assay:^{^[3]}

Cell lines
Mouse macrophage cell line, RAW264.7 cells
Concentrations
3 mM
Incubation Time
6 h
Method
Mouse macrophage cell line, RAW264.7 cells are cultured in DMEM with 10% fetal bovine serum at 37 ℃ in a 5% CO₂ incubator. SiNPs are diluted by DMEM to appropriate concentrations, and an ER stress inhibitor, 4-phenylbutyric acid (4-PBA) is applied (3 mM, 6 h). The SiNPs-treated group is at a concentration of 50 μg/ml in vitro experiments. Similarly, the application of 4-PBA is set up through MTT assay, after SiNPs with or without 4-PBA treatment, cells are harvested for the following measurement. The levels of Bip and CHOP induced by SiNPs are measured.

References

[4] Park HJ, et al. Biochem Pharmacol. 2018 May;151:9-17.

+ Expand

Selleck's 4-PBA (4-Phenylbutyric acid) has been cited by 17 publications

A novel CSN5/CRT O-GlcNAc/ER stress regulatory axis in platinum resistance of epithelial ovarian cancer [ Int J Biol Sci, 2024, 20(4):1279-1296]	PubMed: 38385070
Melatonin protects RPE cells from necroptosis and NLRP3 activation via promoting SERCA2-related intracellular Ca2+ homeostasis [ Phytomedicine, 2024, 135:156088]	PubMed: 39341129
Inhibition of OGFOD1 by FG4592 confers neuroprotection by activating unfolded protein response and autophagy after ischemic stroke [ J Transl Med, 2024, 22(1):248]	PubMed: 38454480
IRE1α arm of unfolded protein response in muscle-specific TGF-β signaling-mediated regulation of muscle cell immunological properties [ Cell Mol Biol Lett, 2023, 28(1):15]	PubMed: 36849929
Porcine reproductive and respiratory syndrome virus infection triggers autophagy via ER stress-induced calcium signaling to facilitate virus replication [ PLoS Pathog, 2023, 19(3):e1011295]	PubMed: 36972295
Routes of Albumin Overload Toxicity in Renal Tubular Epithelial Cells [ Int J Mol Sci, 2023, 24(11)9640]	PubMed: 37298591
Routes of Albumin Overload Toxicity in Renal Tubular Epithelial Cells [ Int J Mol Sci, 2023, 24(11)9640]	PubMed: 37298591
Large HBV Surface Protein-Induced Unfolded Protein Response Dynamically Regulates p27 Degradation in Hepatocellular Carcinoma Progression [ Int J Mol Sci, 2023, 10.3390/ijms241813825]	PubMed: 37762128
Endoplasmic reticulum stress mediates environmental particle-induced inflammatory response in bronchial epithelium [ J Immunotoxicol, 2023, 10.1080/1547691X.2023.2229428]	PubMed: 37417782
Effects of endoplasmic reticulum stress on erectile function in rats with cavernous nerve injury [ Sex Med, 2023, 11(4):qfad050]	PubMed: 37674767

RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.

SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.