4-Octyl Itaconate

Catalog No.S5929 Batch:S592905

Technical Data

Formula	C₁₃H₂₂O₄
Molecular Weight	242.31	CAS No.	3133-16-2
Solubility (25°C)*	In vitro	DMSO	48 mg/mL (198.09 mM)
		Ethanol	48 mg/mL (198.09 mM)
		Water	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	4-Octyl Itaconate activates Nrf2 signaling to inhibit the production of pro-inflammatory cytokines in human macrophages and systemic lupus erythematosus (SLE) patient-derived PBMCs.
In vitro	4-Octyl Itaconate effectively prevents NP cells from degeneration through anti-ROS and anti-inflammation, and the combined use with tBHQ has better effects.^[1]
In vivo	4-Octyl itaconate is a crucial anti-inflammatory metabolite that acts via Nrf2 to limit inflammation and modulate type I interferons.

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines nucleus pulposus (NP) cells Concentrations 5, 10, 15, 20, 25, 30 µM Incubation Time 24 h Method The NP cells are cultured in the 24 well plates with a density of 1.5×10⁴ per well. To test the optimized concentration of tBHQ, 4-Octyl Itaconate, and the mixture of them, ranged concentration of drugs is used to stimulate NP cells for 24 hours. The highest concentration that did not affect the cell viability is applied in our experiment. Besides, we also used Lipopolysaccharides to induce NP cell degeneration.
Animal Study:^{^[2]}	Animal Models C57BL/6 mice Dosages 50 mg/kg Administration i.p.

Cell Assay:^{^[1]}

Cell lines
nucleus pulposus (NP) cells
Concentrations
5, 10, 15, 20, 25, 30 µM
Incubation Time
24 h
Method
The NP cells are cultured in the 24 well plates with a density of 1.5×10⁴ per well. To test the optimized concentration of tBHQ, 4-Octyl Itaconate, and the mixture of them, ranged concentration of drugs is used to stimulate NP cells for 24 hours. The highest concentration that did not affect the cell viability is applied in our experiment. Besides, we also used Lipopolysaccharides to induce NP cell degeneration.

Animal Study:^{^[2]}

Animal Models
C57BL/6 mice
Dosages
50 mg/kg
Administration
i.p.

References

Selleck's 4-Octyl Itaconate has been cited by 2 publications

Induction of autophagy-dependent ferroptosis to eliminate drug-tolerant human retinoblastoma cells [ Cell Death Dis, 2022, 13(6):521]	PubMed: 35654783
The effect of Nrf2 activators tBHQ and 4-octyl itaconate on the nucleus pulposus cell degeneration [ Eur Rev Med Pharmacol Sci, 2021, 25(16):5189-5198]	PubMed: 34486693

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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