4-Octyl Itaconate

Catalog No.S5929 Batch:S592902

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Technical Data

Formula

C13H22O4
Molecular Weight 242.31 CAS No. 3133-16-2
Solubility (25°C)* In vitro DMSO 100 mg/mL (412.69 mM)
Ethanol 48 mg/mL (198.09 mM)
Water Insoluble
In vivo (Add solvents to the product individually and in order)
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O
5.0mg/ml Taking the 1 mL working solution as an example, add 50 μL of 100 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
Clear solution
5% DMSO 95% Corn oil
5.0mg/ml Taking the 1 mL working solution as an example, add 50 μL of 100 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description 4-Octyl Itaconate activates Nrf2 signaling to inhibit the production of pro-inflammatory cytokines in human macrophages and systemic lupus erythematosus (SLE) patient-derived PBMCs.
In vitro

4-Octyl Itaconate effectively prevents NP cells from degeneration through anti-ROS and anti-inflammation, and the combined use with tBHQ has better effects.[1]
In vivo

4-Octyl itaconate is a crucial anti-inflammatory metabolite that acts via Nrf2 to limit inflammation and modulate type I interferons.

Protocol (from reference)

Cell Assay:

[1]
  • Cell lines

    nucleus pulposus (NP) cells

  • Concentrations

    5, 10, 15, 20, 25, 30 µM

  • Incubation Time

    24 h

  • Method

    The NP cells are cultured in the 24 well plates with a density of 1.5×104 per well. To test the optimized concentration of tBHQ, 4-Octyl Itaconate, and the mixture of them, ranged concentration of drugs is used to stimulate NP cells for 24 hours. The highest concentration that did not affect the cell viability is applied in our experiment. Besides, we also used Lipopolysaccharides to induce NP cell degeneration.
Animal Study:

[2]
  • Animal Models

    C57BL/6 mice

  • Dosages

    50 mg/kg

  • Administration

    i.p.

Selleck's 4-Octyl Itaconate has been cited by 2 publications

Induction of autophagy-dependent ferroptosis to eliminate drug-tolerant human retinoblastoma cells [ Cell Death Dis, 2022, 13(6):521] PubMed: 35654783
The effect of Nrf2 activators tBHQ and 4-octyl itaconate on the nucleus pulposus cell degeneration [ Eur Rev Med Pharmacol Sci, 2021, 25(16):5189-5198] PubMed: 34486693

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.