4-HNE (4-Hydroxynonenal)

Catalog No.S9793 Batch:S979301

Technical Data

Formula	C₉H₁₆O₂
Molecular Weight	156.22	CAS No.	75899-68-2
Solubility (25°C)*	In vitro


* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Biological Activity

Description	4-HNE (4-Hydroxynonenal) is one of the major end products of lipid peroxidation and has been widely accepted as an inducer of oxidative stress.
In vitro	4-Hydroxynonenal (4-HNE) is one of the main end products of lipid peroxidation, which induces the expression of heme oxygenase-1 (HO-1), peroxiredoxin-1 and stress protein A170 in an Nrf2-dependent manner in murine macrophages.<sup><a class="sref" href="#s_ref">[2]</a></sup>
Density	0.9 g/mL

Protocol (from reference)

Cell Assay:^{<sup><a class="sref" href="#s_ref">[2]</a></sup>}	Cell lines Mouse Peritoneal Macrophages Concentrations 10 μM Incubation Time 24 h Method Oil Red O Staining: Mouse peritoneal macrophages were incubated for 24 h at 37℃ in 5% CO2 in PRMI 1640 medium containing 10% FCS in the absence or presence of 4-Hydroxynonenal (4-HNE) (10 μM) or moxLDL or oxLDL (200 μg protein/ml). Cells were washed twice, fixed in formalin, and stained with oil red O. The area of lipid-loaded macrophages was measured using a computerized MacSCOPE image analysis system.

Cell Assay:^{<sup><a class="sref" href="#s_ref">[2]</a></sup>}

Cell lines
Mouse Peritoneal Macrophages
Concentrations
10 μM
Incubation Time
24 h
Method
Oil Red O Staining: Mouse peritoneal macrophages were incubated for 24 h at 37℃ in 5% CO2 in PRMI 1640 medium containing 10% FCS in the absence or presence of 4-Hydroxynonenal (4-HNE) (10 μM) or moxLDL or oxLDL (200 μg protein/ml). Cells were washed twice, fixed in formalin, and stained with oil red O. The area of lipid-loaded macrophages was measured using a computerized MacSCOPE image analysis system.

References

Selleck's 4-HNE (4-Hydroxynonenal) has been cited by 1 publication

IL-23 signaling prevents ferroptosis-driven renal immunopathology during candidiasis [ Nat Commun, 2022, 13(1):5545]	PubMed: 36138043

IL-23 signaling prevents ferroptosis-driven renal immunopathology during candidiasis [ Nat Commun, 2022, 13(1):5545]

PubMed: 36138043

RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.

SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.