4-Aminobenzohydrazide

Catalog No.S9874 Batch:S987401

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Technical Data

Formula

C7H9N3O
Molecular Weight 151.17 CAS No. 5351-17-7
Solubility (25°C)* In vitro DMSO 30 mg/mL (198.45 mM)
Water Insoluble
Ethanol Insoluble
In vivo (Add solvents to the product individually and in order)
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O
5.0mg/ml Taking the 1 mL working solution as an example, add 50 μL of 100 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description 4-Aminobenzohydrazide (4-Aminobenzhydrazide, 4-aminobenzoic acid hydrazide, 4-ABAH, Myeloperoxidase Inhibitor I, NSC 640) is an inhibitor of MPO (Myeloperoxidase) enzyme that enhances iNOS induction in MPO-positive cells, but not in MPO-KO cells.
Targets
MPO [1]
In vitro

4-aminobenzohydrazide is an inhibitor of MPO enzyme that enhances iNOS induction in MPO-positive cells, but not in MPO-KO cells.[1]
In vivo

Hamsters treated with ABAH show no significant differences in the percentage of lesions or in the percentage of amoebae damaged compared with the untreated hamsters. ABAH treated mice versus untreated mice show larger abscesses and a decreased percentage of damaged amoebae in these lesion at all stages of evolution.[2]

Protocol (from reference)

Cell Assay:

[1]
  • Cell lines

    bone marrow cells

  • Concentrations

    0.1 μM, 10 μM, 100 μM, 200 μM

  • Incubation Time

    8 h

  • Method

    If MPO suppresses the induction of iNOS by IL, inhibitors of MPO enzyme activity should alleviate this suppression. To test this, bone marrow cells from TgM-POg and MPO-KO mice are incubated with IFNc/LPS for 8 h in the presence of increasing concentrations of 4-aminobenzohydrazide (ABAH).
Animal Study:

[2]
  • Animal Models

    9 week-old Hamsters, 7–10 week-old male BALB/c mice

  • Dosages

    40 mg/kg

  • Administration

    IP

Selleck's 4-Aminobenzohydrazide has been cited by 1 publication

Intratarget Microdosing for Deep Phenotyping of Multiple Drug Effects in the Live Brain [ Front Bioeng Biotechnol, 2022, 10:855755] PubMed: 35372313

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.